MCM6 antibody

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Rabbit anti MCM6

MCM6 antibody recognizes DNA replication licensing factor MCM6, also known as p105MCM. The antibody has been extensively validated for western blotting using whole cell lysates.

MCM6 Antibody with Control Lysate

MCM6 antibody recognizes DNA replication licensing factor MCM6, also known as p105MCM. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00023 WB 100 µl
VPA00023KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human MCM6 antibody recognizes human minichromosome maintenance 6 (MCM6), also known as DNA replication licensing factor or p105MCM. MCM6 is a 821 amino acid nuclear component of the MCM complex containing a single MCM domain.

MCM6 is involved in the initiation and control of eukaryotic DNA replication. Studies show that MCM4 forms a hexameric protein complex with MCM6 and 7, which has DNA helicase activity, and may act as a DNA unwinding enzyme (Kanter et al. 2008). The phosphorylation of this complex by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex (Ishimi et al. 2001). MCM6 has been noted to have elevated expression in Hodgkin's lymphoma (Karimi et al. 2008) and be a predictor of survival in patients with mantle cell lymphoma (Schrader et al. 2005).

Rabbit anti Human MCM6 antibody recognizes human MCM6 as a single band of ~105 kDa in multiple human cell line lysates by western blotting under reducing conditions. It also cross reacts with rodent cell lines tested.

Product Details

Target Species
Human
Western Blotting
Anti MCM6 antibody detects a band of approximately 105 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to a region within human MCM6
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q14566 Related reagents
Entrez Gene
MCM6 Related reagents
GO Terms
GO:0000075 cell cycle checkpoint
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0000216 M/G1 transition of mitotic cell cycle
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006270 DNA-dependent DNA replication initiation
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006350 transcription
GO:0042802 identical protein binding
GO:0045449 regulation of transcription
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of MCM6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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