MCM2 antibody

ISO 9001 Quality Assurance Manufacturing | Print

Anti MCM2 Antibody gallery image 1 — Western blot analysis of HeLa cell lysate (Lane 2), NIH/3T3 cell lysate (Lane 3) and PC12 cell lysate (lane 4), run under reducing conditions and probed with Rabbit anti MCM2 antibody (**AHP2404**) at 1μg/ml. As the secondary antibody HRP conjugated Goat anti Rabbit IgG antibody (STAR208P) was used at 1/10 000. Rabbit anti MCM2 detects a band of approximately 125 kDa.

Anti MCM2 Antibody gallery image 2 — Immunohistochemical analysis of frozen human testis using Rabbit anti MCM2 antibody (**AHP2404**) at 2 μg/ml.

Anti MCM2 Antibody gallery image 3 — Immunohistochemical analysis of formalin fixed paraffin embedded mouse testis using Rabbit anti MCM2 antibody (**AHP2404**) at 2 μg/ml. HRP conjugated Sheep anti Rabbit IgG was used as the secondary antibody.

Anti MCM2 Antibody gallery image 4 — Immunohistochemical analysis of formalin fixed paraffin embedded rat testis using Rabbit anti MCM2 antibody (**AHP2404**) at 2 μg/ml. HRP conjugated Sheep anti Rabbit IgG was used as the secondary antibody

Anti MCM2 Antibody gallery image 5 — Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil using Rabbit anti MCM2 antibody (**AHP2404**) at 2 μg/ml.

Anti MCM2 Antibody gallery image 6 — Immunofluorescence staining of formalin fixed paraffin embedded human tonsil using Rabbit anti MCM2 antibody (**AHP2404**) at 2 μg/ml. As the secondary antibody, Sheep anti Rabbit IgG DyLight®549 conjugated antibody was used. Co-staining was performed with Mouse anti Human CD19 (MCA2454) . Goat anti Mouse IgG (H/L) DyLight 488 conjugated antibody (STAR117D488) was used as the secondary. PureBlu DAPI (1351303) was used as nuclear counterstain (blue).

Anti MCM2 Antibody gallery image 7 — Immunofluorescence staining of formalin fixed paraffin embedded human colon adenocarcinoma using Rabbit anti MCM2 antibody (**AHP2404**) at 2 μg/ml. As the secondary antibody, Sheep anti Rabbit IgG DyLight 549 conjugated antibody was used. Co-staining was performed with Mouse anti Human cytokeratin 18 (MCA1864H). Goat anti mouse IgG (H/L) DyLight 488 conjugated antibody (STAR117D488) was used as the secondary. PureBlu DAPI (1351303) was used as nuclear counterstain (blue).

Filter by Application:

WB C P IF

Rabbit anti MCM2

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: MCM2

Quick Links:

Secondary Antibodies
Applications
Useful Reagents
Storage Information
Batch/Lot Specific Datasheets

Antibody Quality and Performance Guarantee

Unrivaled Technical Support

Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
AHP2404 Datasheet Datasheet SDS SDS	C IF P WB	0.1 ml		Log in	Get Quote
	Log in

Rabbit anti MCM2 antibody recognizes the nuclear protein mini chromosome maintenance protein 2 (MCM2), a putative DNA replicative helicase and member of the MCM family, critical during the initiation of DNA replication.

During the G1 phase of the cell cycle the MCM proteins, part of the pre-replicative complex which assembles at replication origins, undergo phosphorylation by kinases including cyclin dependent kinase (CDK) and Cdc7/Dbf4 (DDK). Phosphorylation of MCM2 occurs on multiple sites, namely Ser27, Ser41, Ser53, Ser108 and Ser139 and results in a conformational change and activation of helicase activity.

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Antiserum Preparation: Antiserum to MCM2 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Peptide corresponding to an N-terminal sequence of human MCM2
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Target Species	Cross Reactivity
Mouse
Rat

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Immunofluorescence	1/100	1/1000
Immunohistology - Frozen	1/100	1/1000
Immunohistology - Paraffin	1/100	1/1000
Western Blotting	1/1000	1/10000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
List Price	Your Price
	Log in
Description	Goat anti Rabbit IgG (H/L):HRP

Description	Product Code	Applications	Pack Size	Your Price
Mouse anti Human CD19	MCA2454	E F* IP P* WB	0.2 mg	Log in	Get Quote
List Price	Your Price
	Log in
Description	Mouse anti Human CD19
PUREBLU™ DAPI	1351303	F IF	250 µg	Log in	Get Quote
List Price	Your Price
	Log in
Description	PUREBLU™ DAPI

UniProt: P49736
Entrez Gene: MCM2
GO Terms: GO:0000075 cell cycle checkpoint; GO:0000082 G1/S transition of mitotic cell cycle; GO:0000084 S phase of mitotic cell cycle; GO:0005515 protein binding; GO:0000216 M/G1 transition of mitotic cell cycle; GO:0000785 chromatin; GO:0046872 metal ion binding; GO:0005524 ATP binding; GO:0005654 nucleoplasm; View More GO Terms; GO:0006270 DNA-dependent DNA replication initiation; GO:0006271 DNA strand elongation involved in DNA replication; GO:0006350 transcription; GO:0045449 regulation of transcription