MCM2 antibody

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Rabbit anti MCM2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2404 C IF P WB 0.1 ml
Rabbit anti MCM2 antibody recognizes the nuclear protein mini chromosome maintenance protein 2 (MCM2), a putative DNA replicative helicase and member of the MCM family, critical during the initiation of DNA replication.

During the G1 phase of the cell cycle the MCM proteins, part of the pre-replicative complex which assembles at replication origins, undergo phosphorylation by kinases including cyclin dependent kinase (CDK) and Cdc7/Dbf4 (DDK). Studies have shown that the phosphorylation of MCM2 occurs on multiple sites, namely Ser27, Ser41, Ser53, Ser108 and Ser139 and that phosphorylation results in a conformational change of the complex and activation of the helicase activity.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to MCM2 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Peptide corresponding to an N-terminal sequence of human MCM2
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch

More Information

UniProt
P49736 Related reagents
Entrez Gene
MCM2 Related reagents
GO Terms
GO:0000075 cell cycle checkpoint
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0005515 protein binding
GO:0000216 M/G1 transition of mitotic cell cycle
GO:0000785 chromatin
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006270 DNA-dependent DNA replication initiation
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006350 transcription
GO:0045449 regulation of transcription
Regulatory
For research purposes only

Applications of MCM2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/100 1/1000
Immunohistology - Frozen 1/100 1/1000
Immunohistology - Paraffin 1/100 1/1000
Western Blotting 1/1000 1/10000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Histar Detection System STAR3000A 50 Tests C P

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
PUREBLU™ DAPI 1351303 250 µg F IF
Mouse anti Human Cytokeratin 18 MCA1864H 5 ml C IF P*
Mouse anti Human CD19 MCA2454 0.2 mg E F* IP P* WB