MCM2 antibody







During the G1 phase of the cell cycle the MCM proteins, part of the pre-replicative complex which assembles at replication origins, undergo phosphorylation by kinases including cyclin dependent kinase (CDK) and Cdc7/Dbf4 (DDK). Studies have shown that the phosphorylation of MCM2 occurs on multiple sites, namely Ser27, Ser41, Ser53, Ser108 and Ser139 and that phosphorylation results in a conformational change of the complex and activation of the helicase activity.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to MCM2 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Peptide corresponding to an N-terminal sequence of human MCM2
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P49736
- Entrez Gene
- MCM2
- GO Terms
- GO:0000075 cell cycle checkpoint
- GO:0000082 G1/S transition of mitotic cell cycle
- GO:0000084 S phase of mitotic cell cycle
- GO:0005515 protein binding
- GO:0000216 M/G1 transition of mitotic cell cycle
- GO:0000785 chromatin
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0005654 nucleoplasm
- GO:0006270 DNA-dependent DNA replication initiation
- GO:0006271 DNA strand elongation involved in DNA replication
- GO:0006350 transcription
- GO:0045449 regulation of transcription
- Regulatory
- For research purposes only
Applications of MCM2 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunofluorescence | 1/100 | 1/1000 | |
Immunohistology - Frozen | 1/100 | 1/1000 | |
Immunohistology - Paraffin | 1/100 | 1/1000 | |
Western Blotting | 1/1000 | 1/10000 |
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Histar Detection System | STAR3000A | C P | 50 Tests | ||
Goat anti Rabbit IgG (H/L):HRP | STAR208P | WB | 2 ml |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse anti Human CD19 | MCA2454 | E F* IP P* WB | 0.2 mg |
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PUREBLU™ DAPI | 1351303 | F IF | 250 µg |
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