Mcl-1 antibody

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Goat anti Human Mcl-1 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2138 E WB 0.1 mg
Goat anti Human Mcl-1 antibody recognises an epitope within the C-Terminal (CT) region of human Mcl-1, otherwise known as Myeloid cell leukaemia sequence 1, a mitochondrial-associated anti-apoptotic member of the Bcl-2 family, which acts as both an enhancer of cell survival (isoform 1), and promoter of cell death (isoform 2).

Mcl-1 is expressed in many different cell lines, and several types of cancer, and blockage of its function, may prove to be a useful therapeutic agent in the treatment of malignancies and inflammatory and infectious diseases.

Goat anti Human Mcl-1 antibody is expected to recognise the longer gene product, isoform 1.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog Expected from Sequence
Rat
Bovine Expected from Sequence
Pig Expected from Sequence
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human Mcl-1 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence C-AGVGAGLAYLIR from the C-Terminal region of Mcl-1 (NP_068779.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q07820 Related reagents
Entrez Gene
MCL1 Related reagents
Regulatory
For research purposes only

Applications of Mcl-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000
Western Blotting 1.0 3.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2138 detects a band of approximately 35kDa in human lung and K562 cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Application Based External Images

Western Blotting

Product Specific References

References for Mcl-1 antibody

  1. Cencic, R. et al. (2013) Modifying chemotherapy response by targeted inhibition of eukaryotic initiation factor 4A.
    Blood Cancer J. 3: e128.