Mcl-1 antibody
Goat anti Human Mcl-1 antibody recognises an epitope within the C-Terminal (CT) region of human Mcl-1, otherwise known as Myeloid cell leukaemia sequence 1, a mitochondrial-associated anti-apoptotic member of the Bcl-2 family, which acts as both an enhancer of cell survival (isoform 1), and promoter of cell death (isoform 2).
Mcl-1 is expressed in many different cell lines, and several types of cancer, and blockage of its function, may prove to be a useful therapeutic agent in the treatment of malignancies and inflammatory and infectious diseases.
Goat anti Human Mcl-1 antibody is expected to recognise the longer gene product, isoform 1.
Mcl-1 is expressed in many different cell lines, and several types of cancer, and blockage of its function, may prove to be a useful therapeutic agent in the treatment of malignancies and inflammatory and infectious diseases.
Goat anti Human Mcl-1 antibody is expected to recognise the longer gene product, isoform 1.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Dog Expected from Sequence Rat Bovine Expected from Sequence Pig Expected from Sequence Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human Mcl-1 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Synthetic peptide sequence C-AGVGAGLAYLIR from the C-Terminal region of Mcl-1 (NP_068779.1).
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
Applications of Mcl-1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/2000 | ||
| Western Blotting | 1.0 | 3.0ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP2138 detects a band of approximately 35kDa in human lung and K562 cell lysates.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Product Specific References
References for Mcl-1 antibody
-
Cencic, R. et al. (2013) Modifying chemotherapy response by targeted inhibition of eukaryotic initiation factor 4A.
Blood Cancer J. 3: e128.
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