Mcl-1 antibody

Goat anti Human Mcl-1 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Mcl-1
Region
(C-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP2138
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg
List Price Your Price
Search for Batch Specific Datasheets

Goat anti Human Mcl-1 antibody recognises an epitope within the C-Terminal (CT) region of human Mcl-1, otherwise known as Myeloid cell leukaemia sequence 1, a mitochondrial-associated anti-apoptotic member of the Bcl-2 family, which acts as both an enhancer of cell survival (isoform 1), and promoter of cell death (isoform 2).

Mcl-1 is expressed in many different cell lines, and several types of cancer, and blockage of its function, may prove to be a useful therapeutic agent in the treatment of malignancies and inflammatory and infectious diseases.

Goat anti Human Mcl-1 antibody is expected to recognise the longer gene product, isoform 1.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog Expected from Sequence
Rat
Bovine Expected from Sequence
Pig Expected from Sequence
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human Mcl-1 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence C-AGVGAGLAYLIR from the C-Terminal region of Mcl-1 (NP_068779.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000
Western Blotting 1.0 3.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2138 detects a band of approximately 35kDa in human lung and K562 cell lysates.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):HRP

References for Mcl-1 antibody

  1. Cencic, R. et al. (2013) Modifying chemotherapy response by targeted inhibition of eukaryotic initiation factor 4A.
    Blood Cancer J. 3: e128.

Western Blotting

Synonyms
Myeloid Cell Leukaemia Sequence 1
RRID
AB_10671454
UniProt
Q07820
Entrez Gene
MCL1
View more products with MCL-1 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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