MARCKS antibody

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Rabbit anti MARCKS

MARCKS antibody recognizes myristoylated alanine-rich C-kinase substrate, also known as myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L), phosphomyristin, or protein kinase C substrate, 80 kDa protein, light chain.

Product Type: PrecisionAb™ Polyclonal
Isotype: Polyclonal IgG
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VPA00204 Datasheet	WB MSDS	100 µl	Log in		Get Quote

Summary
Secondary Antibodies

Rabbit anti Human MARCKS antibody recognizes the myristoylated alanine-rich C-kinase substrate (MARCKS), also known as protein kinase C substrate, 80 kDa protein, light chain, 80K-L protein and PKCSL.

MARCKS is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. MARCKS is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis (provided by RefSeq, Jul 2008).

Rabbit anti Human MARCKS antibody detects a band of 80 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti MARCKS detects a band of approximately 80 kDa in HEK293 cell lysates

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Rabbit Ig fraction prepared by ammonium sulphate precipitation

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

KLH conjugated synthetic peptide between 241-271 amino acids from the C-terminal region of human MARCKS

Instructions For Use

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: As supplied, 12 months from date of despatch.

More Information

UniProt: P29966 Related reagents
Entrez Gene: MARCKS Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0051015 actin filament binding; GO:0005516 calmodulin binding; GO:0006112 energy reserve metabolic process; GO:0050796 regulation of insulin secretion; GO:0015629 actin cytoskeleton
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of MARCKS antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

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