Rabbit anti MAPKAPK2 (pThr334) antibody
recognizes MAP kinase-activated protein kinase 2 (MAPKAPK2), when phosphorylated at threonine 334. MAPKAPK2 belongs to the CAMK serine/threonine protein kinase family and is activated in response to cellular stress and cytokines. MAPKAPK2 is phosphorylated at threonine 222, serine 272 and threonine 334 by MAPK14 (Ben-Levy et al. 1995
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to MAPKAPK2 (pThr334) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding threonine 334 of human MAPKAPK2
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
activation of MAPK activity
MyD88-dependent toll-like receptor signaling pathway
MyD88-independent toll-like receptor signaling pathway
protein serine/threonine kinase activity
signal transducer activity
prostanoid metabolic process
Ras protein signal transduction
Toll signaling pathway
leukotriene biosynthetic process
toll-like receptor 1 signaling pathway
toll-like receptor 2 signaling pathway
toll-like receptor 3 signaling pathway
toll-like receptor 4 signaling pathway
hormone biosynthetic process
innate immune response
nerve growth factor receptor signaling pathway
stress-activated MAPK cascade
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of MAPKAPK2 (pThr334) antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Ben-Levy, R. et al. (1995) Identification of novel phosphorylation sites required for activation of MAPKAP kinase-2.
EMBO J. 14 (23): 5920-30.