MAPKAPK2 (pThr334) antibody
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WB P ResetRabbit anti MAPKAPK2 (pThr334)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- MAPKAPK2 (pThr334)
Antibody Quality and Performance Guarantee
| Rabbit anti MAPKAPK2 (pThr334) antibody recognizes MAP kinase-activated protein kinase 2 (MAPKAPK2), when phosphorylated at threonine 334. MAPKAPK2 belongs to the CAMK serine/threonine protein kinase family and is activated in response to cellular stress and cytokines. MAPKAPK2 is phosphorylated at threonine 222, serine 272 and threonine 334 by MAPK14 (Ben-Levy et al. 1995). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to MAPKAPK2 (pThr334) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding threonine 334 of human MAPKAPK2
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin |  |
1/50 | 1/100 |
| Western Blotting | |
1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Further Reading
-
Ben-Levy, R. et al. (1995) Identification of novel phosphorylation sites required for activation of MAPKAP kinase-2.
EMBO J. 14 (23): 5920-30.
- UniProt
- P49137
- Entrez Gene
- MAPKAPK2
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0005515 protein binding
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0005829 cytosol
- GO:0004674 protein serine/threonine kinase activity
- GO:0004871 signal transducer activity
- GO:0005524 ATP binding
- GO:0005654 nucleoplasm
- View More GO Terms
- GO:0006692 prostanoid metabolic process
- GO:0007265 Ras protein signal transduction
- GO:0008063 Toll signaling pathway
- GO:0019370 leukotriene biosynthetic process
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0042446 hormone biosynthetic process
- GO:0045087 innate immune response
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0051403 stress-activated MAPK cascade
AHP2646
141603
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