MAPK14 (pThr180) antibody
Rabbit anti MAPK14 (pThr180) antibody recognizes mitogen-activated protein kinase 14 (MAPK14), also known as cytokine suppressive anti-inflammatory drug-binding protein, MAX-interacting protein 2 or MAP kinase p38 alpha, when phosphorylated at threonine 180.
MAPK14 is a serine threonine kinase and a member of the MAP kinase family. MAPK14 signaling plays a key role in the regulation of multiple processes including cell differentiation and proliferation.
MAPK14 is phosphorylated at threonine 180 and tyrosine 182 by MAP2K3 and MAP2K6. Phosphorylation at threonine 180 and tyrosine 182 activates MAPK14 leading to phosphorylation of downstream targets such as transcription factors (Raingeaud et al. 1995).
MAPK14 is a serine threonine kinase and a member of the MAP kinase family. MAPK14 signaling plays a key role in the regulation of multiple processes including cell differentiation and proliferation.
MAPK14 is phosphorylated at threonine 180 and tyrosine 182 by MAP2K3 and MAP2K6. Phosphorylation at threonine 180 and tyrosine 182 activates MAPK14 leading to phosphorylation of downstream targets such as transcription factors (Raingeaud et al. 1995).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to MAPK14 (pThr180) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding threonine 180 of human MAPK14
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q16539
- Entrez Gene
- MAPK14
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0005515 protein binding
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0005829 cytosol
- GO:0004707 MAP kinase activity
- GO:0004708 MAP kinase kinase activity
- GO:0005524 ATP binding
- GO:0005654 nucleoplasm
- GO:0006928 cellular component movement
- GO:0006935 chemotaxis
- GO:0007265 Ras protein signal transduction
- GO:0008063 Toll signaling pathway
- GO:0010670 positive regulation of oxygen and reactive oxygen species metabolic process
- GO:0016071 mRNA metabolic process
- GO:0030168 platelet activation
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0042692 muscle cell differentiation
- GO:0042770 signal transduction in response to DNA damage
- GO:0045087 innate immune response
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0051090 regulation of transcription factor activity
- GO:0051149 positive regulation of muscle cell differentiation
- GO:0051403 stress-activated MAPK cascade
- GO:0071479 cellular response to ionizing radiation
- GO:0090400 stress-induced premature senescence
- Regulatory
- For research purposes only
Applications of MAPK14 (pThr180) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Raingeaud, J. et al. (1995) Pro-inflammatory cytokines and environmental stress cause p38 mitogen-activated protein kinase activation by dual phosphorylation on tyrosine and threonine.
J Biol Chem. 270 (13): 7420-6.
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