Rabbit anti MAPK14 (pThr180) antibody
recognizes mitogen-activated protein kinase 14 (MAPK14), also known as cytokine suppressive anti-inflammatory drug-binding protein, MAX-interacting protein 2 or MAP kinase p38 alpha, when phosphorylated at threonine 180.
MAPK14 is a serine threonine kinase and a member of the MAP kinase family. MAPK14 signaling plays a key role in the regulation of multiple processes including cell differentiation and proliferation.
MAPK14 is phosphorylated at threonine 180 and tyrosine 182 by MAP2K3 and MAP2K6. Phosphorylation at threonine 180 and tyrosine 182 activates MAPK14 leading to phosphorylation of downstream targets such as transcription factors (Raingeaud et al. 1995
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to MAPK14 (pThr180) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding threonine 180 of human MAPK14
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
activation of MAPK activity
MyD88-dependent toll-like receptor signaling pathway
MyD88-independent toll-like receptor signaling pathway
MAP kinase activity
MAP kinase kinase activity
cellular component movement
Ras protein signal transduction
Toll signaling pathway
positive regulation of oxygen and reactive oxygen species metabolic process
mRNA metabolic process
toll-like receptor 1 signaling pathway
toll-like receptor 2 signaling pathway
toll-like receptor 3 signaling pathway
toll-like receptor 4 signaling pathway
muscle cell differentiation
signal transduction in response to DNA damage
innate immune response
nerve growth factor receptor signaling pathway
regulation of transcription factor activity
positive regulation of muscle cell differentiation
stress-activated MAPK cascade
cellular response to ionizing radiation
stress-induced premature senescence
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of MAPK14 (pThr180) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Raingeaud, J. et al. (1995) Pro-inflammatory cytokines and environmental stress cause p38 mitogen-activated protein kinase activation by dual phosphorylation on tyrosine and threonine.
J Biol Chem. 270 (13): 7420-6.