MAPK1 antibody
Rabbit anti Human MAPK1 antibody recognizes MAP kinase 1, also known as mitogen-activated protein kinase 1 or MAP kinase isoform p42.
The protein encoded by MAPK1 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for MAPK1 (provided by RefSeq, Jul 2008).
Rabbit anti Human MAPK1 antibody detects bands at 42 and 44 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The protein encoded by MAPK1 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for MAPK1 (provided by RefSeq, Jul 2008).
Rabbit anti Human MAPK1 antibody detects bands at 42 and 44 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti MAPK1 antibody recognizes bands of approximately 42 and 44 kDa in C2C12 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Immunogen
- Synthetic peptide directed towards the C terminal region of human MAPK1
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P28482
- Entrez Gene
- MAPK1
- GO Terms
- GO:0000186 activation of MAPKK activity
- GO:0000187 activation of MAPK activity
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0003677 DNA binding
- GO:0005829 cytosol
- GO:0004707 MAP kinase activity
- GO:0005524 ATP binding
- GO:0005654 nucleoplasm
- GO:0006350 transcription
- GO:0006917 induction of apoptosis
- GO:0007049 cell cycle
- GO:0007173 epidermal growth factor receptor signaling pathway
- GO:0007265 Ras protein signal transduction
- GO:0007268 synaptic transmission
- GO:0007411 axon guidance
- GO:0008063 Toll signaling pathway
- GO:0008286 insulin receptor signaling pathway
- GO:0008353 RNA polymerase II carboxy-terminal domain kinase activity
- GO:0030168 platelet activation
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0044419 interspecies interaction between organisms
- GO:0045087 innate immune response
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0051090 regulation of transcription factor activity
- GO:0051403 stress-activated MAPK cascade
- GO:0070371 ERK1 and ERK2 cascade
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of MAPK1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | WB | 2 ml |
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