The MAP3K12 gene encodes a member of the serine/threonine protein kinase family. This kinase contains a leucine-zipper domain and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation. Alternatively spliced transcript variants encoding different proteins have been described (provided by RefSeq, Jul 2010).
Rabbit anti Human MAP3K12 antibody detects a band of 97 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
- Target Species
- Western Blotting
- Anti MAP3K12 detects a band of approximately 97 kDa in HEK293 cell lysate
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- KLH-conjugated synthetic peptide corresponding to aa 828-859 of human MAP3K12
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- GO:0005886 plasma membrane
- GO:0000287 magnesium ion binding
- GO:0005829 cytosol
- GO:0004709 MAP kinase kinase kinase activity
- GO:0005524 ATP binding
- GO:0005624 membrane fraction
- GO:0007254 JNK cascade
- GO:0016572 histone phosphorylation
- GO:0018105 peptidyl-serine phosphorylation
- GO:0018107 peptidyl-threonine phosphorylation
- GO:0019901 protein kinase binding
- GO:0042803 protein homodimerization activity
- GO:0046777 protein autophosphorylation
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- For research purposes only
Applications of MAP3K12 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Goat anti Rabbit IgG (H/L):HRP||STAR208P||WB||2 ml|