MAP3K12 antibody

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Rabbit anti MAP3K12

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00377
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human MAP3K12 antibody recognizes MAP3K12, also known as MEKK12, MAPK-upstream kinase, mitogen-activated protein kinase kinase kinase 12, leucine zipper protein kinase, mixed lineage kinase and protein kinase MUK.

The MAP3K12 gene encodes a member of the serine/threonine protein kinase family. This kinase contains a leucine-zipper domain and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation. Alternatively spliced transcript variants encoding different proteins have been described (provided by RefSeq, Jul 2010).

Rabbit anti Human MAP3K12 antibody detects a band of 97 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti MAP3K12 detects a band of approximately 97 kDa in HEK293 cell lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 828-859 of human MAP3K12

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q12852
Entrez Gene
MAP3K12
GO Terms
GO:0005886 plasma membrane
GO:0000287 magnesium ion binding
GO:0005829 cytosol
GO:0004709 MAP kinase kinase kinase activity
GO:0005524 ATP binding
GO:0005624 membrane fraction
GO:0007254 JNK cascade
GO:0016572 histone phosphorylation
GO:0018105 peptidyl-serine phosphorylation
GO:0018107 peptidyl-threonine phosphorylation
GO:0019901 protein kinase binding
GO:0042803 protein homodimerization activity
GO:0046777 protein autophosphorylation
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of MAP3K12 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml