Rabbit anti Human Mannosidase II antibody recognizes human mannosidase II, also known as golgi alpha mannosidase II, Mannosidase alpha class 2A member 1 or Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase. Mannosidase II is a 1144 amino acid ~135 kDa protein involved in the biosynthesis of complex N-glycans. Mannosidase II is is a single pass type II transmembrane glycoprotein located on the luminal side of golgi membranes and is a useful marker for the golgi apparatus.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to mannosidase II were raised by repeated immunisations of rabbits with highly purified antigen.
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to membrane
mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity
mannose metabolic process
zinc ion binding
protein N-linked glycosylation via asparagine
post-translational protein modification
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Mannosidase II antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Application Based External Images
Product Specific References
References for Mannosidase II antibody
Herbert, S.P. et al. (2005) Cytosolic phospholipase A2-alpha mediates endothelial cell proliferation and is inactivated by association with the Golgi apparatus.
Mol Biol Cell. 16: 3800-9.
Desai, P. et al. (2008) Localization of herpes simplex virus type 1 UL37 in the Golgi complex requires UL36 but not capsid structures.
J Virol. 82:11354-61.
Tangemo, C. et al. (2011) A novel laser nanosurgery approach supports de novo Golgi biogenesis in mammalian cells.
J Cell Sci. 124: 978-87.
Herbert, S.P. ert al. (2007) The confluence-dependent interaction of cytosolic phospholipase A2-alpha with annexin A1 regulates endothelial cell prostaglandin E2 generation.
J Biol Chem. 282: 34468-78.
Rybakin, V. et al. (2008) Molecular mechanism underlying the association of Coronin-7 with Golgi membranes.
Cell Mol Life Sci. 65: 2419-30.
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