Mannosidase II antibody

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Rabbit anti Human Mannosidase II

Product Type
Polyclonal Antibody
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
0.1 ml loader

Rabbit anti Human Mannosidase II antibody recognizes human mannosidase II, also known as golgi alpha mannosidase II, Mannosidase alpha class 2A member 1 or Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase. Mannosidase II is a 1144 amino acid ~135 kDa protein involved in the biosynthesis of complex N-glycans. Mannosidase II is is a single pass type II transmembrane glycoprotein located on the luminal side of golgi membranes and is a useful marker for the golgi apparatus.

Product Details

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to mannosidase II were raised by repeated immunisations of rabbits with highly purified antigen.
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)

Storage Information

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0000139 Golgi membrane
GO:0016021 integral to membrane
GO:0004559 alpha-mannosidase activity
GO:0004572 mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity
GO:0030246 carbohydrate binding
GO:0006013 mannose metabolic process
GO:0008270 zinc ion binding
GO:0018279 protein N-linked glycosylation via asparagine
GO:0043687 post-translational protein modification
For research purposes only

Applications of Mannosidase II antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/100 1/400
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Application Based External Images


Product Specific References

References for Mannosidase II antibody

  1. Herbert, S.P. et al. (2005) Cytosolic phospholipase A2-alpha mediates endothelial cell proliferation and is inactivated by association with the Golgi apparatus.
    Mol Biol Cell. 16: 3800-9.
  2. Desai, P. et al. (2008) Localization of herpes simplex virus type 1 UL37 in the Golgi complex requires UL36 but not capsid structures.
    J Virol. 82:11354-61.
  3. Tangemo, C. et al. (2011) A novel laser nanosurgery approach supports de novo Golgi biogenesis in mammalian cells.
    J Cell Sci. 124: 978-87.
  4. Herbert, S.P. ert al. (2007) The confluence-dependent interaction of cytosolic phospholipase A2-alpha with annexin A1 regulates endothelial cell prostaglandin E2 generation.
    J Biol Chem. 282: 34468-78.
  5. Rybakin, V. et al. (2008) Molecular mechanism underlying the association of Coronin-7 with Golgi membranes.
    Cell Mol Life Sci. 65: 2419-30.

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