Mannosidase II antibody
Rabbit anti Human Mannosidase II antibody recognizes human mannosidase II, also known as golgi alpha mannosidase II, Mannosidase alpha class 2A member 1 or Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase. Mannosidase II is a 1144 amino acid kDa protein involved in the biosynthesis of complex N-glycans. Mannosidase II is is a single pass type II transmembrane glycoprotein located on the luminal side of golgi membranes and is a useful marker for the golgi apparatus.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Rat Monkey
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to mannosidase II were raised by repeated immunisations of rabbits with highly purified antigen.
- Preservative Stabilisers
0.09% Sodium Azide
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- Q16706 Related reagents
- Entrez Gene
- MAN2A1 Related reagents
- GO Terms
- GO:0000139 Golgi membrane
- GO:0016021 integral to membrane
- GO:0004559 alpha-mannosidase activity
- GO:0004572 mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity
- GO:0030246 carbohydrate binding
- GO:0006013 mannose metabolic process
- GO:0008270 zinc ion binding
- GO:0018279 protein N-linked glycosylation via asparagine
- GO:0043687 post-translational protein modification
- For research purposes only
Applications of Mannosidase II antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||1/500|
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Sheep anti Rabbit IgG:Biotin||2AB02B||1 ml||C E P WB|
|Goat anti Rabbit IgG (Fc):Biotin||STAR121B||1 mg||E WB|
|Goat anti Rabbit IgG (Fc):FITC||STAR121F||1 mg||F|
|Goat anti Rabbit IgG (Fc):HRP||STAR121P||1 mg||E WB|
|Goat anti Rabbit IgG (H/L):HRP||STAR124P||1 mg||C E WB|
|Sheep anti Rabbit IgG:FITC||STAR34B||1 mg||C F|
|Sheep anti Rabbit IgG:RPE||STAR35A||1 ml||F|
|Sheep anti Rabbit IgG:DyLight®488||STAR36D488GA||0.1 mg||F IF WB|
|Sheep anti Rabbit IgG:DyLight®549||STAR36D549GA||0.1 mg||F IF|
|Sheep anti Rabbit IgG:DyLight®649||STAR36D649GA||0.1 mg||F IF|
|Sheep anti Rabbit IgG:DyLight®680||STAR36D680GA||0.1 mg||F IF WB|
|Sheep anti Rabbit IgG:Dylight®800||STAR36D800GA||0.1 mg||F IF WB|
Product Specific References
References for Mannosidase II antibody
Herbert, S.P. et al. (2005) Cytosolic phospholipase A2-alpha mediates endothelial cell proliferation and is inactivated by association with the Golgi apparatus.
Mol Biol Cell. 16: 3800-9.
Desai, P. et al. (2008) Localization of herpes simplex virus type 1 UL37 in the Golgi complex requires UL36 but not capsid structures.
J Virol. 82:11354-61.
Tangemo, C. et al. (2011) A novel laser nanosurgery approach supports de novo Golgi biogenesis in mammalian cells.
J Cell Sci. 124: 978-87.
Herbert, S.P. ert al. (2007) The confluence-dependent interaction of cytosolic phospholipase A2-alpha with annexin A1 regulates endothelial cell prostaglandin E2 generation.
J Biol Chem. 282: 34468-78.
Rybakin, V. et al. (2008) Molecular mechanism underlying the association of Coronin-7 with Golgi membranes.
Cell Mol Life Sci. 65: 2419-30.