Manic Fringe antibody
Rabbit anti Manic Fringe antibody recognizes beta-1,3-N-acetylglucosaminyltransferase manic fringe, also known as MFNG. It belongs to the β3N-acteylglucosaminyl-transferases family that modulates the Notch signaling pathway activity (Haines and Irvine 2003, Kakuda and Haltiwanger 2017). In claudin-low breast cancer cell lines, manic fringe has been shown to act as an oncogene by Notch-mediated induction of PIK3CG (Zhang et al. 2015).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to Manic Fringe was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- 0.42% Potassium phosphate
0.87% Sodium chloride - Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
30% Glycerol - Immunogen
- KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Manic Fringe
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O00587
- Entrez Gene
- MFNG
- GO Terms
- GO:0005615 extracellular space
- GO:0007389 pattern specification process
- GO:0030173 integral to Golgi membrane
- GO:0033829 O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity
- Regulatory
- For research purposes only
Applications of Manic Fringe antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti Manic Fringe antibody detects a band of approximately 36 kDa in HepG2 cell lysate
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
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