Manic Fringe antibody

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Rabbit anti Manic Fringe

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2709
Datasheet
WB
MSDS
50 µl loader

Rabbit anti Manic Fringe antibody recognizes beta-1,3-N-acetylglucosaminyltransferase manic fringe, also known as MFNG. It belongs to the β3N-acteylglucosaminyl-transferases family that modulates the Notch signaling pathway activity (Haines and Irvine 2003, Kakuda and Haltiwanger 2017). In claudin-low breast cancer cell lines, manic fringe has been shown to act as an oncogene by Notch-mediated induction of PIK3CG (Zhang et al. 2015).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to Manic Fringe was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
0.42% Potassium phosphate
0.87% Sodium chloride
Preservative Stabilisers
0.01% Sodium Azide (NaN3)
30% Glycerol
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Manic Fringe

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
O00587
Entrez Gene
MFNG
GO Terms
GO:0005615 extracellular space
GO:0007389 pattern specification process
GO:0030173 integral to Golgi membrane
GO:0033829 O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity
Regulatory
For research purposes only

Applications of Manic Fringe antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti Manic Fringe antibody detects a band of approximately 36 kDa in HepG2 cell lysate

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg