Manic Fringe antibody

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Goat anti Human Manic Fringe

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2266 E P* 0.1 mg
Goat anti Human Manic Fringe antibody recognises Manic Fringe. Manic fringe (MFNG), Lunatic Fringe (LFNG) and Radical Fringe (RFNG) are members of the mammalian fringe gene family, which exhibit fucose-specific beta1,3 N-acetylglucosaminyltransferase activity. These proteins act as modulators during ligand activation of the Notch signaling pathway, through the elongation of Notch fucose residues.

The Notch signaling pathway is an evolutionarily conserved pathway in multicellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell hematopoiesis, thymic T cell development, and both tumor progression and suppression.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human MFNG was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography using the immunizing peptide
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence C-NRPIHASEPQPHNRT from the internal region of Manic Fringe (NP_002396.2; NP_001159815.1)
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
O00587 Related reagents
Entrez Gene
MFNG Related reagents
GO Terms
GO:0005615 extracellular space
GO:0007389 pattern specification process
GO:0030173 integral to Golgi membrane
GO:0033829 O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity
Regulatory
For research purposes only

Applications of Manic Fringe antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/8000
Immunohistology - Paraffin 1 2.0 4.0ug/ml
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Tris/EDTA buffer pH 9.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human Cerebral Cortex

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Product Specific References

Further Reading

  1. Bray, S.J. (2006) Notch signalling: a simple pathway becomes complex.
    Nat Rev Mol Cell Biol. 7 (9): 678-89.