MADD antibody

Rabbit anti MADD

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
MADD

Product Code Applications Pack Size List Price Your Price Qty
AHP480
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C WB 0.1 mg loader
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loader
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Rabbit anti MADD antibody recognises the apoptosis related protein designated MADD (MAP kinase - activating death domain protein). MADD is associated with Type I TNF receptor via their death domains.

In Western blots this antibody detects MADD as a ~200-220 kDa protein.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human MADD were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
Synthetic peptide from C-terminus of human MADD.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Western Blotting 1/250 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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Further Reading

  1. Schievella, A.R. et al. (1997) MADD, a novel death domain protein that interacts with the type 1 tumor necrosis factor receptor and activates mitogen-activated protein kinase.
    J Biol Chem. 272 (18): 12069-75.
  2. Yoshimura, S. et al. (2010) Family-wide characterization of the DENN domain Rab GDP-GTP exchange factors.
    J Cell Biol. 191: 367-81.

Synonyms
Map kinase-activating Death Domain Protein
RRID
AB_2297098
UniProt
Q8WXG6
Entrez Gene
MADD
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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