MADD antibody
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WB IC ResetRabbit anti MADD
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- MADD
Antibody Quality and Performance Guarantee
| Rabbit anti MADD antibody recognises the apoptosis related protein designated MADD (MAP kinase - activating death domain protein). MADD is associated with Type I TNF receptor via their death domains. In Western blots this antibody detects MADD as a ~200-220 kDa protein. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human MADD were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide - Immunogen
- Synthetic peptide from C-terminus of human MADD.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen |  |
||
| Western Blotting | |
1/250 | 1/500 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Description | Sheep anti Rabbit IgG:FITC | ||||||
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | Log in | |||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
Further Reading
-
Schievella, A.R. et al. (1997) MADD, a novel death domain protein that interacts with the type 1 tumor necrosis factor receptor and activates mitogen-activated protein kinase.
J Biol Chem. 272 (18): 12069-75. -
Yoshimura, S. et al. (2010) Family-wide characterization of the DENN domain Rab GDP-GTP exchange factors.
J Cell Biol. 191: 367-81.
- Synonyms
- Map kinase-activating Death Domain Protein
- RRID
- AB_2297098
- UniProt
- Q8WXG6
- Entrez Gene
- MADD
AHP480
155203
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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