MAD2 antibody
Rabbit anti Human MAD2 antibody recognizes the mitotic spindle assembly checkpoint protein MAD2A, also known as MAD2 (mitotic arrest deficient, yeast, homolog)-like 1, MAD2-like protein 1, mitotic arrest deficient 2-like protein 1 or mitotic spindle assembly checkpoint protein MAD2A.
MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14 (provided by RefSeq, Jul 2008).
Rabbit anti Human MAD2 antibody detects a band of 22 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14 (provided by RefSeq, Jul 2008).
Rabbit anti Human MAD2 antibody detects a band of 22 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
<50% Glycerol - Preservative Stabilisers
- 0.01% Thiomersal
<50% Glycerol - Immunogen
- Carrier-protein conjugated synthetic peptide encompassing part of the C terminal region of human MAD2
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q13257 Related reagents
- Entrez Gene
- MAD2L1 Related reagents
- GO Terms
- GO:0000236 mitotic prometaphase
- GO:0000777 condensed chromosome kinetochore
- GO:0005829 cytosol
- GO:0005634 nucleus
- GO:0007094 mitotic cell cycle spindle assembly checkpoint
- GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
- GO:0042803 protein homodimerization activity
- GO:0043066 negative regulation of apoptosis
- GO:0048471 perinuclear region of cytoplasm
- GO:0051301 cell division
- GO:0060564 negative regulation of mitotic anaphase-promoting complex activity
- GO:0090267 positive regulation of mitotic cell cycle spindle assembly checkpoint
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of MAD2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
- Western Blotting
- Rabbit anti MAD2 detects a band of approximately 22 kDa in HEK293 cell lysates
- Western Blotting
- Rabbit anti MAD2 detects a band of approximately 22 kDa in HEK293 cell lysates
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
