MAD2 antibody

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Rabbit anti MAD2

MAD2 Antibody with Control Lysate

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00732 WB 100 µl
VPA00732KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human MAD2 antibody recognizes the mitotic spindle assembly checkpoint protein MAD2A, also known as MAD2 (mitotic arrest deficient, yeast, homolog)-like 1, MAD2-like protein 1, mitotic arrest deficient 2-like protein 1 or mitotic spindle assembly checkpoint protein MAD2A.

MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14 (provided by RefSeq, Jul 2008).

Rabbit anti Human MAD2 antibody detects a band of 22 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
<50% Glycerol
Preservative Stabilisers
0.01% Thiomersal
<50% Glycerol
Immunogen
Carrier-protein conjugated synthetic peptide encompassing part of the C terminal region of human MAD2

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q13257 Related reagents
Entrez Gene
MAD2L1 Related reagents
GO Terms
GO:0000236 mitotic prometaphase
GO:0000777 condensed chromosome kinetochore
GO:0005829 cytosol
GO:0005634 nucleus
GO:0007094 mitotic cell cycle spindle assembly checkpoint
GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0042803 protein homodimerization activity
GO:0043066 negative regulation of apoptosis
GO:0048471 perinuclear region of cytoplasm
GO:0051301 cell division
GO:0060564 negative regulation of mitotic anaphase-promoting complex activity
GO:0090267 positive regulation of mitotic cell cycle spindle assembly checkpoint
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of MAD2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Rabbit anti MAD2 detects a band of approximately 22 kDa in HEK293 cell lysates
Western Blotting
Rabbit anti MAD2 detects a band of approximately 22 kDa in HEK293 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB