MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14 (provided by RefSeq, Jul 2008).
Rabbit anti Human MAD2 antibody detects a band of 22 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
- Target Species
- Western Blotting
- Rabbit anti MAD2 detects a band of approximately 22 kDa in HEK293 cell lysates
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Carrier-protein conjugated synthetic peptide encompassing part of the C terminal region of human MAD2
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- GO:0000236 mitotic prometaphase
- GO:0000777 condensed chromosome kinetochore
- GO:0005829 cytosol
- GO:0005634 nucleus
- GO:0007094 mitotic cell cycle spindle assembly checkpoint
- GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
- GO:0042803 protein homodimerization activity
- GO:0043066 negative regulation of apoptosis
- GO:0048471 perinuclear region of cytoplasm
- GO:0051301 cell division
- GO:0060564 negative regulation of mitotic anaphase-promoting complex activity
- GO:0090267 positive regulation of mitotic cell cycle spindle assembly checkpoint
- PrecisionAb is a trademark of Bio-Rad Laboratories
- For research purposes only
Applications of MAD2 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Goat anti Rabbit IgG (H/L):HRP||STAR208P||WB||2 ml|