MAD2 antibody

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Rabbit anti MAD2

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00732
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human MAD2 antibody recognizes the mitotic spindle assembly checkpoint protein MAD2A, also known as MAD2 (mitotic arrest deficient, yeast, homolog)-like 1, MAD2-like protein 1, mitotic arrest deficient 2-like protein 1 or mitotic spindle assembly checkpoint protein MAD2A.

MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14 (provided by RefSeq, Jul 2008).

Rabbit anti Human MAD2 antibody detects a band of 22 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Rabbit anti MAD2 detects a band of approximately 22 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
<50% Glycerol
Immunogen
Carrier-protein conjugated synthetic peptide encompassing part of the C terminal region of human MAD2

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
Q13257
Entrez Gene
MAD2L1
GO Terms
GO:0000236 mitotic prometaphase
GO:0000777 condensed chromosome kinetochore
GO:0005829 cytosol
GO:0005634 nucleus
GO:0007094 mitotic cell cycle spindle assembly checkpoint
GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0042803 protein homodimerization activity
GO:0043066 negative regulation of apoptosis
GO:0048471 perinuclear region of cytoplasm
GO:0051301 cell division
GO:0060564 negative regulation of mitotic anaphase-promoting complex activity
GO:0090267 positive regulation of mitotic cell cycle spindle assembly checkpoint
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of MAD2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml