M-CSF antibody

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Anti M-CSF Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with M-CSF antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 5 second exposure. Arrow points to M-CSF (molecular weight 70 kDa)

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Rabbit anti M-CSF

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: M-CSF

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VPA00353 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human M-CSF antibody recognizes macrophage colony-stimulating factor 1 (M-CSF), also known as lanimostim.

Encoded by the CSF1 gene, M-CSF is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants (provided by RefSeq, Sep 2011).

Rabbit anti Human M-CSF antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti M-CSF detects a band of approximately 70 kDa in HEK293 cell lysate.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Ig fraction - liquid
Preparation: Rabbit Ig fraction prepared by ammonium sulphate precipitation.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: KLH-conjugated synthetic peptide corresponding to aa 230-257 of human M-CSF
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P09603
Entrez Gene: CSF1
GO Terms: GO:0005886 plasma membrane; GO:0001954 positive regulation of cell-matrix adhesion; GO:0003006 developmental process involved in reproduction; GO:0016021 integral to membrane; GO:0005125 cytokine activity; GO:0005157 macrophage colony-stimulating factor receptor binding; GO:0005615 extracellular space; GO:0008083 growth factor activity; GO:0008283 cell proliferation; View More GO Terms; GO:0010628 positive regulation of gene expression; GO:0010744 positive regulation of macrophage derived foam cell differentiation; GO:0030225 macrophage differentiation; GO:0030316 osteoclast differentiation; GO:0030335 positive regulation of cell migration; GO:0032270 positive regulation of cellular protein metabolic process; GO:0032946 positive regulation of mononuclear cell proliferation; GO:0042117 monocyte activation; GO:0042803 protein homodimerization activity; GO:0043235 receptor complex; GO:0045651 positive regulation of macrophage differentiation; GO:0045657 positive regulation of monocyte differentiation; GO:0045860 positive regulation of protein kinase activity; GO:0048471 perinuclear region of cytoplasm