M-CSF antibody

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Rabbit anti M-CSF

Rabbit anti human M-CSF antibody recognizes macrophage colony-stimulating factor 1, also known as lanimostim. This antibody has been extensively validated for western blotting using whole cell lysates.

M-CSF Antibody with Control Lysate

Rabbit anti human M-CSF antibody recognizes macrophage colony-stimulating factor 1, also known as lanimostim. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00353 WB 100 µl
VPA00353KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human M-CSF antibody recognizes macrophage colony-stimulating factor 1 (M-CSF), also known as lanimostim.

Encoded by the CSF1 gene, M-CSF is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants (provided by RefSeq, Sep 2011).

Rabbit anti Human M-CSF antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ig fraction - liquid
Product Form
Ig fraction - liquid
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation
Preparation
20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 230-257 of human M-CSF

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P09603 Related reagents
Entrez Gene
CSF1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0001954 positive regulation of cell-matrix adhesion
GO:0003006 developmental process involved in reproduction
GO:0016021 integral to membrane
GO:0005125 cytokine activity
GO:0005157 macrophage colony-stimulating factor receptor binding
GO:0005615 extracellular space
GO:0008083 growth factor activity
GO:0008283 cell proliferation
GO:0010628 positive regulation of gene expression
GO:0010744 positive regulation of macrophage derived foam cell differentiation
GO:0030225 macrophage differentiation
GO:0030316 osteoclast differentiation
GO:0030335 positive regulation of cell migration
GO:0032270 positive regulation of cellular protein metabolic process
GO:0032946 positive regulation of mononuclear cell proliferation
GO:0042117 monocyte activation
GO:0042803 protein homodimerization activity
GO:0043235 receptor complex
GO:0045651 positive regulation of macrophage differentiation
GO:0045657 positive regulation of monocyte differentiation
GO:0045860 positive regulation of protein kinase activity
GO:0048471 perinuclear region of cytoplasm
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of M-CSF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti M-CSF detects a band of approximately 70 kDa in HEK293 cell lysate
Western Blotting
Anti M-CSF detects a band of approximately 70 kDa in HEK293 cell lysate
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB