M-CSF antibody
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P ResetRabbit anti Human M-CSF
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- M-CSF
Antibody Quality and Performance Guarantee
| Rabbit anti Human M-CSF antibody recognizes human M-CSF (Macrophage Colony-Stimulating Factor), a potent lineage restricted haematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which plays a key role in the regulation of cellular differentiation, proliferation, and the survival of blood monocytes, tissue macrophages and their progenitor cells. Along with sRANKL/CD254/OPGL, M-CSF is essential for osteoclastogenesis, acting through the tyrosine kinase receptor c-fms (colony stimulating factor 1 receptor), and facilitates haematopoietic recovery following bone marrow transplantation. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antiserum to human M-CSF were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human M-CSF
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 2.0ug/ml |
| Functional Assays | |
0.02 | 0.03ug/ml |
| Immunohistology - Paraffin 1 | |
||
| Western Blotting | |
0.1 | 0.2ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- This antibody may be used under either reducing or non-reducing western blotting conditions with PHP220 as the positive control.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Description | Sheep anti Rabbit IgG:FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Recombinant Human M-CSF | PHP220 | E FN WB | 10 µg |
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| List Price | Your Price | ||||||
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| Description | Recombinant Human M-CSF | ||||||
- Synonyms
- Macrophage COLONY-STIMULATING Factor
- RRID
- AB_2085068
- UniProt
- P09603
- Entrez Gene
- CSF1
- GO Terms
- GO:0005886 plasma membrane
- GO:0001954 positive regulation of cell-matrix adhesion
- GO:0003006 developmental process involved in reproduction
- GO:0016021 integral to membrane
- GO:0005125 cytokine activity
- GO:0005157 macrophage colony-stimulating factor receptor binding
- GO:0005615 extracellular space
- GO:0008083 growth factor activity
- GO:0008283 cell proliferation
- View More GO Terms
- GO:0010628 positive regulation of gene expression
- GO:0010744 positive regulation of macrophage derived foam cell differentiation
- GO:0030225 macrophage differentiation
- GO:0030316 osteoclast differentiation
- GO:0030335 positive regulation of cell migration
- GO:0032270 positive regulation of cellular protein metabolic process
- GO:0032946 positive regulation of mononuclear cell proliferation
- GO:0042117 monocyte activation
- GO:0042803 protein homodimerization activity
- GO:0043235 receptor complex
- GO:0045651 positive regulation of macrophage differentiation
- GO:0045657 positive regulation of monocyte differentiation
- GO:0045860 positive regulation of protein kinase activity
- GO:0048471 perinuclear region of cytoplasm
AHP1915
160602
160823
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