Lysine-Specific Histone Demethylase antibody
Rabbit anti Human lysine-specific histone demethylase antibody recognizes lysine-specific histone demethylase (LSD1), a ubiquitously expressed nuclear protein belonging to the flavin monoamine oxidase family and containing a single SWIRM domain.
Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is an amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction. Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues, e.g. H3-K4, are associated with active transcription.
Rabbit anti Human lysine-specific histone demethylase antibody recognizes a band of ~115 kDa in multiple cell lines by western blotting under reducing conditions. This antibody is expected to recognize both described isoforms of lysine-specific histone demethylase.
Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is an amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction. Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues, e.g. H3-K4, are associated with active transcription.
Rabbit anti Human lysine-specific histone demethylase antibody recognizes a band of ~115 kDa in multiple cell lines by western blotting under reducing conditions. This antibody is expected to recognize both described isoforms of lysine-specific histone demethylase.
Product Details
- Target Species
- Human
- Western Blotting
- Anti lysine-specific histone demethylase antibody detects a band of approximately 115 kDa in Jurkat cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Peptide corresponding corresponding to a 17 amino acid sequence from the N-terminus of human LSD1
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- O60341
- Entrez Gene
- KDM1A
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Lysine-Specific Histone Demethylase antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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