Lysine-Specific Histone Demethylase antibody
Rabbit anti Lysine-Specific Histone Demethylase
Lysine-specific histone demethylase antibody recognizes lysine-specific histone demethylase 1A, also known as BRAF35-HDAC complex protein BHC110 or flavin-containing amine oxidase domain-containing protein 2. The antibody has been extensively validated for western blotting using whole cell lysates.
Lysine-Specific Histone Demethylase Antibody with Control Lysate
Lysine-specific histone demethylase antibody recognizes lysine-specific histone demethylase 1A, also known as BRAF35-HDAC complex protein BHC110 or flavin-containing amine oxidase domain-containing protein 2. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00009 | WB | 100 µl | ||||
| VPA00009KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human lysine-specific histone demethylase antibody recognizes lysine-specific histone demethylase (LSD1), a ubiquitously expressed nuclear protein belonging to the flavin monoamine oxidase family and containing a single SWIRM domain.
Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is an amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction. Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues, e.g. H3-K4, are associated with active transcription.
Rabbit anti Human lysine-specific histone demethylase antibody recognizes a band of ~115 kDa in multiple cell lines by western blotting under reducing conditions. This antibody is expected to recognize both described isoforms of lysine-specific histone demethylase.
Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is an amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction. Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues, e.g. H3-K4, are associated with active transcription.
Rabbit anti Human lysine-specific histone demethylase antibody recognizes a band of ~115 kDa in multiple cell lines by western blotting under reducing conditions. This antibody is expected to recognize both described isoforms of lysine-specific histone demethylase.
Product Details
- Target Species
- Human
- Western Blotting
- Anti lysine-specific histone demethylase antibody detects a band of approximately 115 kDa in Jurkat cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Peptide corresponding corresponding to a 17 amino acid sequence from the N-terminus of human LSD1
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- O60341 Related reagents
- Entrez Gene
- KDM1A Related reagents
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Lysine-Specific Histone Demethylase antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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