Lysine-Specific Histone Demethylase antibody

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Rabbit anti Lysine-Specific Histone Demethylase

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00009
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human lysine-specific histone demethylase antibody recognizes lysine-specific histone demethylase (LSD1), a ubiquitously expressed nuclear protein belonging to the flavin monoamine oxidase family and containing a single SWIRM domain.

Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is an amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction. Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues, e.g. H3-K4, are associated with active transcription.

Rabbit anti Human lysine-specific histone demethylase antibody recognizes a band of ~115 kDa in multiple cell lines by western blotting under reducing conditions. This antibody is expected to recognize both described isoforms of lysine-specific histone demethylase.

Product Details

Target Species
Human
Western Blotting
Anti lysine-specific histone demethylase antibody detects a band of approximately 115 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Peptide corresponding corresponding to a 17 amino acid sequence from the N-terminus of human LSD1

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
O60341 Related reagents
Entrez Gene
KDM1A Related reagents
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Lysine-Specific Histone Demethylase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

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