Lyn antibody

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Rabbit anti Lyn

Lyn Antibody with Control Lysate

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00605 WB 100 µl
VPA00605KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human Lyn antibody recognizes the tyrosine-protein kinase Lyn, also known as lck/Yes-related novel protein tyrosine kinase, v-yes-1 or Yamaguchi sarcoma viral related oncogene homolog.

The LYN gene encodes a tyrosine protein kinase, which maybe involved in the regulation of mast cell degranulation, and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for LYN (provided by RefSeq, Jul 2011).

Rabbit anti Human Lyn antibody detects bands of 53 and 56 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Rabbit anti Lyn detects bands of approximately 53 and 56 kDa in Ramos cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
KLH conjugated synthetic peptide between amino acids 43 - 77 of human Lyn
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Ramos lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P07948 Related reagents
Entrez Gene
LYN Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0005829 cytosol
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0004716 receptor signaling protein tyrosine kinase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005794 Golgi apparatus
GO:0006468 protein phosphorylation
GO:0006974 response to DNA damage stimulus
GO:0008284 positive regulation of cell proliferation
GO:0009725 response to hormone stimulus
GO:0030168 platelet activation
GO:0030218 erythrocyte differentiation
GO:0031295 T cell costimulation
GO:0042531 positive regulation of tyrosine phosphorylation of STAT protein
GO:0044419 interspecies interaction between organisms
GO:0045121 membrane raft
GO:0048471 perinuclear region of cytoplasm
GO:0050900 leukocyte migration
GO:0051272 positive regulation of cellular component movement
GO:0070304 positive regulation of stress-activated protein kinase signaling cascade
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Lyn antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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