Lyn antibody

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AHP2494
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Rabbit anti Lyn antibody recognizes Lyn, also known as LYN proto-oncogene or JTK8. Lyn is a member of the Src family of protein tyrosine kinases. Lyn plays a vital role in myeloid differentiation (Harder et al. 2001).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to Lyn was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Recombinant human Lyn
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/50 1/200
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti Lyn antibody detects a band of approximately 59 kDa in A431 cell lysates and mouse tissue lysates under reducing conditions

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UniProt
P07948
Entrez Gene
LYN
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0005829 cytosol
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0004716 receptor signaling protein tyrosine kinase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005794 Golgi apparatus
GO:0006468 protein phosphorylation
GO:0006974 response to DNA damage stimulus
GO:0008284 positive regulation of cell proliferation
GO:0009725 response to hormone stimulus
GO:0030168 platelet activation
GO:0030218 erythrocyte differentiation
GO:0031295 T cell costimulation
GO:0042531 positive regulation of tyrosine phosphorylation of STAT protein
GO:0044419 interspecies interaction between organisms
GO:0045121 membrane raft
GO:0048471 perinuclear region of cytoplasm
GO:0050900 leukocyte migration
GO:0051272 positive regulation of cellular component movement
GO:0070304 positive regulation of stress-activated protein kinase signaling cascade

AHP2494

141460

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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