LXR Beta antibody

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Rabbit anti LXR Beta

Rabbit anti-human LXR beta antibody recognizes oxysterols receptor LXR-beta, also known as liver X nuclear receptor beta, nuclear orphan receptor LXR-beta, nuclear receptor NER, or steroid hormone-nuclear receptor NER.

LXR Beta Antibody with Control Lysate

Product Type: PrecisionAb™ Polyclonal
Isotype: Polyclonal IgG
Format: Purified

Product Code	Applications	Pack Size
VPA00584	WB	100 µl
VPA00584KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Rabbit anti Human LXR beta antibody recognizes the oxysterols receptor LXR beta, also known as ubiquitously-expressed nuclear receptor, nuclear receptor NER, steroid hormone nuclear receptor NER or nuclear receptor subfamily 1 group H member 2.

The liver X receptors, LXRA and LXRB, form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. The inducible LXRA gene is highly expressed in liver, adrenal gland, intestine, adipose tissue, macrophages, lung, and kidney, whereas LXRB is ubiquitously expressed. Ligand-activated LXRs form obligate heterodimers with retinoid X receptors and regulate expression of target genes containing LXR response elements (Korf et al. 2009) (supplied by OMIM, Jan 2010).

Rabbit anti Human LXR beta antibody detects a band of 63 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti LXR beta detects a band of approximately 63 kDa in A549 cell lysates
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography
Preparation: 20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
2% Sucrose
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
2% Sucrose
Immunogen: Synthetic peptide directed towards the middle region of human LXR beta
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg A549 lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch
Shelf Life: As supplied, 12 months from date of despatch

More Information

UniProt: P55055 Related reagents
Entrez Gene: NR1H2 Related reagents
GO Terms: GO:0003707 steroid hormone receptor activity; GO:0005737 cytoplasm; GO:0005654 nucleoplasm; GO:0008270 zinc ion binding; GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter; GO:0010745 negative regulation of macrophage derived foam cell differentiation; GO:0010867 positive regulation of triglyceride biosynthetic process; GO:0010875 positive regulation of cholesterol efflux; GO:0010887 negative regulation of cholesterol storage; GO:0032270 positive regulation of cellular protein metabolic process; GO:0032369 negative regulation of lipid transport; GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor; GO:0043565 sequence-specific DNA binding; GO:0045723 positive regulation of fatty acid biosynthetic process; GO:0048550 negative regulation of pinocytosis; GO:0051006 positive regulation of lipoprotein lipase activity
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of LXR Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Rabbit IgG (H/L):HRP	STAR208P	2 ml	WB
Goat anti Rabbit IgG (H/L):HRP	STAR208P	2 ml	WB

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