LOX-1 antibody

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Sheep anti Human LOX-1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP952 C F IF IP WB 50 µg
Sheep anti LOX-1 antibody recognizes the type II membrane glycoprotein LOX-1 (lectin-like oxidized LDL receptor-1), a 40-50kDa class E scavenger receptor, expressed by endothelial cells, macrophages, activated platelets and smooth muscle cells. LOX-1 binds to a wide range of ligands, including oxidized low-density lipoprotein (oxLDL), hypochlorite modified high-density lipoprotein (HDL), aged/apoptotic cells, activated platelets and bacteria, reflecting its versatile physiological functions.

AHP952 will detect LOX-1 on transfected cells and in recombinant protein reconstitution assays. LOX-1 levels are low in normal resting human tissues so may fall below detectable levels.

Expression of the LOX-1 gene is upregulated by oxLDL and the binding of LOX-1 to oxLDL results in the activation of NF-kappaB. Furthermore, LOX-1 antibodies have been shown to suppress the oxidized HDL (oxHDL) activation of NF-KappaB in endothelial cells, suggesting that this activation may be due to the binding of oxHDL to LOX-1.

Angiotensin II and the inflammatory cytokine Tumour Necrosis Factor alpha (TNFalpha) also invoke an increase in LOX-1 gene expression and studies have focused on its role in endothelial dysfunction (Chen, M. et al.)(Sawamura, T. et al.) and inflammatory diseases such as atherosclerosis (Murphy, J.E. et al.) and rheumatoid arthritis, as well as its affect on CD40/CD40L signaling in both atherosclerosis and human coronary artery endothelial cells (HCAECs) (Li, D. et al. ).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human LOX-1 were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Recombinant human extracellular domain LOX-1 protein from bacteria.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P78380 Related reagents
Entrez Gene
OLR1 Related reagents
GO Terms
GO:0007596 blood coagulation
GO:0005576 extracellular region
GO:0005887 integral to plasma membrane
GO:0005529 sugar binding
GO:0005624 membrane fraction
GO:0006508 proteolysis
GO:0006954 inflammatory response
GO:0008015 blood circulation
GO:0050900 leukocyte migration
Regulatory
For research purposes only

Applications of LOX-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation Neat
Western Blotting 1/10 1/100
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology
For the best results Bio-Rad recommend HISTAR detection kits..
Western Blotting
Sheep anti LOX-1 antibody detects intact &'126;50 kDa LOX-1 and also a prominent ~32 kDa proteolytic fragment in western blotting.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Application Based External Images

Immunofluorescence

Product Specific References

References for LOX-1 antibody

  1. Murphy, J.E. et al. (2006) LOX-1 scavenger receptor mediates calcium-dependent recognition of phosphatidylserine and apoptotic cells.
    Biochem J. 393 (Pt 1): 107-15.

Further Reading

  1. Chen, M. et al. (2002) LOX-1, the receptor for oxidized low-density lipoprotein identified from endothelial cells: implications in endothelial dysfunction and atherosclerosis.
    Pharmacol Ther. 95 (1): 89-100.
  2. Li, D. et al. (2003) LOX-1, an oxidized LDL endothelial receptor, induces CD40/CD40L signaling in human coronary artery endothelial cells.
    Arterioscler Thromb Vasc Biol. 23 (5): 816-21.
  3. Morawietz, H. et al. (1999) Angiotensin II induces LOX-1, the human endothelial receptor for oxidized low-density lipoprotein.
    Circulation. 100 (9): 899-902.
  4. Sawamura, T. et al. (1997) An endothelial receptor for oxidized low-density lipoprotein.
    Nature. 386 (6620): 73-7.
  5. Yoshida, H. et al. (1998) Identification of the lectin-like receptor for oxidized low-density lipoprotein in human macrophages and its potential role as a scavenger receptor.
    Biochem J. 334 ( Pt 1): 9-13.