Rabbit anti LIMK2 (pThr505) antibody
recognizes LIM domain kinase 2 (LIMK2), when phosphorylated at threonine 505. LIMK2 is a member of the serine/threonine protein kinase family involved in the regulation of actin cytoskeleton reorganization. LIMK2 is phosphorylated at threonine 505 by Cdc42/Rac-PAK1, 2, 4 and ROCK1, increasing LIMK2 activity and leading to stabilization of the actin cytoskeleton (Petrilli et al. 2014
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to LIMK2 (pThr505) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding threonine 505 of human LIMK2
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
protein serine/threonine kinase activity
zinc ion binding
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of LIMK2 (pThr505) antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Petrilli, A. et al. (2014) LIM domain kinases as potential therapeutic targets for neurofibromatosis type 2.
Oncogene. 33 (27): 3571-82.
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