Rabbit anti LIMK1 (pThr508) antibody
recognizes LIM domain kinase 1 (LIMK1), when phosphorylated at threonine 505. LIMK1 is a member of the serine/threonine protein kinase family involved in the regulation of actin cytoskeleton reorganization. LIMK1 is phosphorylated at threonine 508 by Cdc42/Rac-PAK1, 2, 4 and ROCK1, increasing LIMK1 kinase activity and leads to stabilization of the actin cytoskeleton (Petrilli et al. 2014
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to LIMK1 (pThr508) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding threonine 508 of human LIMK1
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
actin cytoskeleton organization
protein serine/threonine kinase activity
Rho protein signal transduction
zinc ion binding
heat shock protein binding
positive regulation of actin filament bundle assembly
positive regulation of axon extension
negative regulation of ubiquitin-protein ligase activity
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of LIMK1 (pThr508) antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Petrilli, A. et al. (2014) LIM domain kinases as potential therapeutic targets for neurofibromatosis type 2.
Oncogene. 33 (27): 3571-82.