LGR5 antibody

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Rabbit anti LGR5

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2742
Datasheet
WB
MSDS
50 µl loader

Rabbit anti LGR5 antibody recognizes Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5), also known as G-protein coupled receptor 49, G-protein coupled receptor 67 and G-protein coupled receptor HG38. Lgr5 is a Wnt target gene and marks proliferative stem cells in the small intestine and colon (Barker et al. 2007) and stomach (Barker et al. 2010). LGR5 promotes tumor proliferation and invasion (Xi et al. 2014, Parry and Engh 2014). LGR5 expression is increased with glioblastoma progression (Nakata et al. 2013, Zhang et al. 2018).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to LGR5 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
Immunogen
A synthetic peptide corresponding to amino acids 689-719 of human LGR5

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
O75473
Entrez Gene
LGR5
GO Terms
GO:0005887 integral to plasma membrane
GO:0016500 protein-hormone receptor activity
Regulatory
For research purposes only

Applications of LGR5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti LGR5 antibody detects a band of approximately 100 kDa in HeLa and HepG2 cell lysate and mouse skeletal muscle tissue lysate under reducing conditions

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg