Leukemia Inhibitory Factor antibody

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Rabbit anti Leukemia Inhibitory Factor

Rabbit anti human leukemia inhibitory factor antibody recognizes leukemia inhibitory factor, also known as LIF, D factor, cholinergic differentiation factor, differentiation inhibitory activity, or melanoma-derived LPL inhibitor. This antibody has been extensively validated for western blotting.

Leukemia Inhibitory Factor Antibody with Control Lysate

Rabbit anti human leukemia inhibitory factor antibody recognizes leukemia inhibitory factor, also known as LIF, D factor, cholinergic differentiation factor, differentiation inhibitory activity, differentiation-inducing factor, differentiation-stimulating factor, or hepatocyte-stimulating factor III

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00279 WB 100 µl
VPA00279KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human leukemia inhibitory factor antibody recognizes leukemia inhibitory factor, also known as LIF, D factor, cholinergic differentiation factor, differentiation-stimulating factor, hepatocyte-stimulating factor III and melanoma-derived LPL inhibitor.

Leukemia inhibitory factor gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for LIF (provided by RefSeq, Mar 2012).

Rabbit anti Human leukemia inhibitory factor antibody detects a band of 25 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti leukemia inhibitory factor detects a band of approximately 25 kDa in Jurkat cell lysate
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 72-101 of human leukamia inhibitory factor
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P15018 Related reagents
Entrez Gene
LIF Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005146 leukemia inhibitory factor receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0008284 positive regulation of cell proliferation
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0033141 positive regulation of peptidyl-serine phosphorylation of STAT protein
GO:0042511 positive regulation of tyrosine phosphorylation of Stat1 protein
GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
GO:0043410 positive regulation of MAPKKK cascade
GO:0045651 positive regulation of macrophage differentiation
GO:0046888 negative regulation of hormone secretion
GO:0048861 leukemia inhibitory factor signaling pathway
GO:0072108 positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
GO:0072307 regulation of metanephric nephron tubule epithelial cell differentiation
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Leukemia Inhibitory Factor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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