Leptin Receptor antibody

Goat anti Human Leptin Receptor

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Leptin Receptor

Product Code Applications Pack Size List Price Your Price Qty
AHP1396
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg
List Price Your Price
Search for Batch Specific Datasheets

Goat anti Human Leptin receptor antibody recognizes the human leptin receptor, designated CD295, a type 1 transmembrane glycoprotein and member of the cytokine receptor family, which binds with high affinity to the adipocyte-specific hormone leptin, an obesity factor involved in the regulation of adipose-tissue mass/body weight through hypothalamic effects.

The effects of leptin through binding to the long form of CD295 (LRb), are mediated via the JAK/STAT3 signalling pathway, and are vital for the regulation of the normal physiology of many cells, including food intake, lipid metabolism and energy expenditure. CD295-leptin signalling is also involved in the differential regulation of naive and memory T cell proliferation and promotes the release of pro-inflammatory cytokines, including interferon and interleukin-2.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human leptin receptor was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence C-TQDDIEKHQSDAG from the internal region of leptin receptor (NP_002294.2; NP_001003679.1; NP_001003680.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/16000
Western Blotting 1.0 3.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1396 detects a band of approximately 150kDa in human brain cell lysates.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):HRP

Further Reading

  1. Tartaglia, L.A. (1997) The leptin receptor.
    J Biol Chem. 272 (10): 6093-6.

Synonyms
CD295
RRID
AB_2136358
UniProt
P48357
Entrez Gene
LEPR
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004896 cytokine receptor activity
GO:0006112 energy reserve metabolic process
View more products with LEPTIN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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