Leptin Receptor antibody

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Anti Human Leptin Receptor Antibody thumbnail image 2

Anti Human Leptin Receptor Antibody gallery image 1 — Western blotting analysis of Mouse (A) and Rat (B) brain lysates (35μg protein in RIPA buffer) probed with Goat anti Human leptin receptor antibody (**AHP1396**) at 0.1μgml^-1 . Primary incubation was 1 hour. Signal was detected by chemiluminescence.

Anti Human Leptin Receptor Antibody gallery image 2 — Western blotting analysis of human cerebral cortex lysate (35μg protein in RIPA buffer) probed with Goat anti Human leptin receptor antibody (**AHP1396**) at 1.0μgml^-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence

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Goat anti Human Leptin Receptor

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: Leptin Receptor

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References (1)
Applications
Storage Information
Batch/Lot Specific Datasheets

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Product Code Applications Pack Size List Price Your Price Qty

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AHP1396 Datasheet Datasheet SDS SDS	E WB	0.1 mg		Log in	Get Quote
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Goat anti Human Leptin receptor antibody recognizes the human leptin receptor, designated CD295, a type 1 transmembrane glycoprotein and member of the cytokine receptor family, which binds with high affinity to the adipocyte-specific hormone leptin, an obesity factor involved in the regulation of adipose-tissue mass/body weight through hypothalamic effects.

The effects of leptin through binding to the long form of CD295 (LRb), are mediated via the JAK/STAT3 signalling pathway, and are vital for the regulation of the normal physiology of many cells, including food intake, lipid metabolism and energy expenditure. CD295-leptin signalling is also involved in the differential regulation of naive and memory T cell proliferation and promotes the release of pro-inflammatory cytokines, including interferon and interleukin-2.

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Dog Expected from Sequence; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Antiserum Preparation: Antiserum to human leptin receptor was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution: TRIS buffered saline
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin
Immunogen: Peptide sequence C-TQDDIEKHQSDAG from the internal region of leptin receptor (NP_002294.2; NP_001003679.1; NP_001003680.1).
Approx. Protein Concentrations: IgG concentration 0.5mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Target Species	Cross Reactivity
Dog	Expected from Sequence

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA			1/16000
Western Blotting		1.0	3.0ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP1396 detects a band of approximately 150kDa in human brain cell lysates.

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