Leptin Receptor antibody

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Goat anti Human Leptin Receptor

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1396 E WB 0.1 mg
Goat anti Human Leptin receptor antibody recognizes the human leptin receptor, designated CD295, a type 1 transmembrane glycoprotein and member of the cytokine receptor family, which binds with high affinity to the adipocyte-specific hormone leptin, an obesity factor involved in the regulation of adipose-tissue mass/body weight through hypothalamic effects.

The effects of leptin through binding to the long form of CD295 (LRb), are mediated via the JAK/STAT3 signalling pathway, and are vital for the regulation of the normal physiology of many cells, including food intake, lipid metabolism and energy expenditure. CD295-leptin signalling is also involved in the differential regulation of naive and memory T cell proliferation and promotes the release of pro-inflammatory cytokines, including interferon and interleukin-2.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human leptin receptor was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence C-TQDDIEKHQSDAG from the internal region of leptin receptor (NP_002294.2; NP_001003679.1; NP_001003680.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P48357 Related reagents
Entrez Gene
LEPR Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004896 cytokine receptor activity
GO:0006112 energy reserve metabolic process
Regulatory
For research purposes only

Applications of Leptin Receptor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/16000
Western Blotting 1.0 3.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1396 detects a band of approximately 150kDa in human brain cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Product Specific References

Further Reading

  1. Tartaglia, L.A. (1997) The leptin receptor.
    J Biol Chem. 272 (10): 6093-6.