Leptin antibody

Rabbit anti Leptin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Leptin

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AHP2493
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
P WB 50 µl loader
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loader

Rabbit anti leptin antibody recognizes leptin, also known as LEP or OBS. Leptin is a hormone made by adipose cells. Leptin binds to receptors present on the arcuate nucleus of the hypothalamus.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to leptin was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Recombinant human leptin
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/50 1/100
Western Blotting 1/200 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti leptin antibody detects a band of approximately 19 kDa in HEK293 cell lysate under reducing conditions

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UniProt
P41159
Entrez Gene
LEP
GO Terms
GO:0005615 extracellular space
GO:0006112 energy reserve metabolic process
GO:0008343 adult feeding behavior
GO:0032099 negative regulation of appetite

AHP2493

141459

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with LEPTIN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products