LDHB antibody
Goat anti LDHB
Goat anti-human LDHB antibody recognizes L-lactate dehydrogenase B chain, also known as LDH heart subunit, epididymis secretory protein Li 281, lactate dehydrogenase H chain or renal carcinoma antigen NY-REN-46.
LDHB Antibody with Control Lysate
Goat anti-human LDHB antibody recognizes L-lactate dehydrogenase B chain, also known as LDH heart subunit, epididymis secretory protein Li 281, lactate dehydrogenase H chain or renal carcinoma antigen NY-REN-46.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00568 | WB | 100 µl | ||||
| VPA00568KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Goat anti Human LDHB antibody recognizes the L-lactate dehydrogenase B chain, also known as LDH heart subunit, epididymis secretory protein Li 281, lactate dehydrogenase H chain or renal carcinoma antigen NY-REN-46.
The LDHB gene encodes an enzyme which catalyzes the reversible conversion of lactate, pyruvate, NAD and NADH, in the glycolytic pathway. Mutations in LDHB are associated with Lactate dehydrogenase B deficiency (LDHBD). Pseudogenes have been identified on the X chromosome and on chromosome. Multiple alternatively spliced variants, encoding the same protein, have been identified (provided by RefSeq, Mar 2010).
Goat anti Human LDHB antibody detects a band of 37 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The LDHB gene encodes an enzyme which catalyzes the reversible conversion of lactate, pyruvate, NAD and NADH, in the glycolytic pathway. Mutations in LDHB are associated with Lactate dehydrogenase B deficiency (LDHBD). Pseudogenes have been identified on the X chromosome and on chromosome. Multiple alternatively spliced variants, encoding the same protein, have been identified (provided by RefSeq, Mar 2010).
Goat anti Human LDHB antibody detects a band of 37 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti LDHB detects a band of approximately 37 kDa in A549 cell lysates.
- Product Form
- Purified IgG - liquid.
- Product Form
- Purified IgG - liquid.
- Preparation
- Goat polyclonal antibody purified by affinity chromatography.
- Preparation
- 20μl Goat polyclonal antibody purified by affinity chromatography.
- Buffer Solution
- TRIS buffered saline.
- Buffer Solution
- TRIS buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin. - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin. - Immunogen
- Peptide sequence C-NARGLTSVINQKLK
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol.
Positive Control Lysate
- Lysate Composition
- 400μg A549 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P07195 Related reagents
- Entrez Gene
- LDHB Related reagents
- GO Terms
- GO:0004459 L-lactate dehydrogenase activity
- GO:0005829 cytosol
- GO:0006090 pyruvate metabolic process
- GO:0006096 glycolysis
- GO:0055114 oxidation-reduction process
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only.
Applications of LDHB antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB | ||
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB |
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