LDHB antibody

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Goat anti LDHB

Goat anti-human LDHB antibody recognizes L-lactate dehydrogenase B chain, also known as LDH heart subunit, epididymis secretory protein Li 281, lactate dehydrogenase H chain or renal carcinoma antigen NY-REN-46.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00568
Datasheet
WB
MSDS
100 µl

Goat anti Human LDHB antibody recognizes the L-lactate dehydrogenase B chain, also known as LDH heart subunit, epididymis secretory protein Li 281, lactate dehydrogenase H chain or renal carcinoma antigen NY-REN-46.

The LDHB gene encodes an enzyme which catalyzes the reversible conversion of lactate, pyruvate, NAD and NADH, in the glycolytic pathway. Mutations in LDHB are associated with Lactate dehydrogenase B deficiency (LDHBD). Pseudogenes have been identified on the X chromosome and on chromosome. Multiple alternatively spliced variants, encoding the same protein, have been identified (provided by RefSeq, Mar 2010).

Goat anti Human LDHB antibody detects a band of 37 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti LDHB detects a band of approximately 37 kDa in A549 cell lysates.
Product Form
Purified IgG - liquid.
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution
TRIS buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin.
Immunogen
Peptide sequence C-NARGLTSVINQKLK
Instructions For Use
Please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P07195 Related reagents
Entrez Gene
LDHB Related reagents
GO Terms
GO:0004459 L-lactate dehydrogenase activity
GO:0005829 cytosol
GO:0006090 pyruvate metabolic process
GO:0006096 glycolysis
GO:0055114 oxidation-reduction process
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only.

Applications of LDHB antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader
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