LDHB antibody

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Anti LDHB Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with PSMB9 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 9 second exposure. Arrow points to PSMB9 (molecular weight 23 kDa).

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Goat anti LDHB

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: LDHB

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Goat anti Human LDHB antibody recognizes the L-lactate dehydrogenase B chain, also known as LDH heart subunit, epididymis secretory protein Li 281, lactate dehydrogenase H chain or renal carcinoma antigen NY-REN-46.

The LDHB gene encodes an enzyme which catalyzes the reversible conversion of lactate, pyruvate, NAD and NADH, in the glycolytic pathway. Mutations in LDHB are associated with Lactate dehydrogenase B deficiency (LDHBD). Pseudogenes have been identified on the X chromosome and on chromosome. Multiple alternatively spliced variants, encoding the same protein, have been identified (provided by RefSeq, Mar 2010).

Goat anti Human LDHB antibody detects a band of 37 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti LDHB detects a band of approximately 37 kDa in A549 cell lysates.
Product Form: Purified IgG - liquid.
Preparation: Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution: TRIS buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin.
Immunogen: Peptide sequence C-NARGLTSVINQKLK
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Donkey anti Sheep/Goat IgG:HRP	STAR88P	C E P WB	1 ml	Log in	Get Quote
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Description	Donkey anti Sheep/Goat IgG:HRP

UniProt: P07195
Entrez Gene: LDHB
GO Terms: GO:0004459 L-lactate dehydrogenase activity; GO:0005829 cytosol; GO:0006090 pyruvate metabolic process; GO:0006096 glycolysis; GO:0055114 oxidation-reduction process