Lck (pTyr416)/FYN (pTyr416) antibody
H2O2 Treatment: Cells were cultured in serum free media for 16-20 hours before treatment with H2O2 (2mM) for 15 minutes
UV Treatment: Cells were treated with UV for 15-30 minutes
Rabbit anti LCK (pTyr416)/FYN (pTyr416) antibody recognizes LCK also known as lymphocyte cell-specific protein-tyrosine kinase, when phosphorylated at tyrosine 416 and FYN also known as Src-like kinase, when phosphorylated at tyrosine 416 FYN.
LCK and FYN are members of the Src family of tyrosine kinases that are involved in the regulation of numerous processes including differentiation and proliferation. Autophosphorylation of LCK and FYN at tyrosine 416 increases kinase activity via an open confirmation of the LCK and FYN which makes substrates more accessible (Boggon and Eck 2004, Irtegun et al. 2004).
LCK and FYN are members of the Src family of tyrosine kinases that are involved in the regulation of numerous processes including differentiation and proliferation. Autophosphorylation of LCK and FYN at tyrosine 416 increases kinase activity via an open confirmation of the LCK and FYN which makes substrates more accessible (Boggon and Eck 2004, Irtegun et al. 2004).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to LCK (pTyr416)/FYN (pTyr416) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding tyrosine 416 of human LCK and tyrosine 416 of human FYN
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch
More Information
- UniProt
- P06239 Related reagents
- P06241 Related reagents
- Entrez Gene
- LCK Related reagents
- FYN Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0001948 glycoprotein binding
- GO:0005829 cytosol
- GO:0004715 non-membrane spanning protein tyrosine kinase activity
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0005768 endosome
- GO:0006468 protein phosphorylation
- GO:0006816 calcium ion transport
- GO:0007243 intracellular protein kinase cascade
- GO:0007411 axon guidance
- GO:0007612 learning
- GO:0007631 feeding behavior
- GO:0042802 identical protein binding
- GO:0016032 viral reproduction
- GO:0030168 platelet activation
- GO:0031295 T cell costimulation
- GO:0044419 interspecies interaction between organisms
- GO:0050690 regulation of defense response to virus by virus
- GO:0050852 T cell receptor signaling pathway
- GO:0050900 leukocyte migration
- Regulatory
- For research purposes only
Applications of Lck (pTyr416)/FYN (pTyr416) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot™ Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
Product Specific References
Further Reading
-
Boggon, T.J. & Eck, M.J. (2004) Structure and regulation of Src family kinases.
Oncogene. 23 (48): 7918-27. -
Irtegun, S. et al. (2013) Tyrosine 416 is phosphorylated in the closed, repressed conformation of c-Src.
PLoS One. 8 (7): e71035.
