Rabbit anti LCK (pTyr416)/FYN (pTyr416) antibody
recognizes LCK also known as lymphocyte cell-specific protein-tyrosine kinase, when phosphorylated at tyrosine 416 and FYN also known as Src-like kinase, when phosphorylated at tyrosine 416 FYN.
LCK and FYN are members of the Src family of tyrosine kinases that are involved in the regulation of numerous processes including differentiation and proliferation. Autophosphorylation of LCK and FYN at tyrosine 416 increases kinase activity via an open confirmation of the LCK and FYN which makes substrates more accessible (Boggon and Eck 2004
, Irtegun et al. 2004
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to LCK (pTyr416)/FYN (pTyr416) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding tyrosine 416 of human LCK and tyrosine 416 of human FYN
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
non-membrane spanning protein tyrosine kinase activity
metal ion binding
calcium ion transport
intracellular protein kinase cascade
identical protein binding
T cell costimulation
interspecies interaction between organisms
regulation of defense response to virus by virus
T cell receptor signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Lck (pTyr416)/FYN (pTyr416) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Boggon, T.J. & Eck, M.J. (2004) Structure and regulation of Src family kinases.
Oncogene. 23 (48): 7918-27.
Irtegun, S. et al. (2013) Tyrosine 416 is phosphorylated in the closed, repressed conformation of c-Src.
PLoS One. 8 (7): e71035.
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