Lck (pTyr416)/FYN (pTyr416) antibody

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Rabbit anti Lck (pTyr416)/FYN (pTyr416)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2641 WB 50 µl
Rabbit anti LCK (pTyr416)/FYN (pTyr416) antibody recognizes LCK also known as lymphocyte cell-specific protein-tyrosine kinase, when phosphorylated at tyrosine 416 and FYN also known as Src-like kinase, when phosphorylated at tyrosine 416 FYN.

LCK and FYN are members of the Src family of tyrosine kinases that are involved in the regulation of numerous processes including differentiation and proliferation. Autophosphorylation of LCK and FYN at tyrosine 416 increases kinase activity via an open confirmation of the LCK and FYN which makes substrates more accessible (Boggon and Eck 2004, Irtegun et al. 2004).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to LCK (pTyr416)/FYN (pTyr416) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding tyrosine 416 of human LCK and tyrosine 416 of human FYN

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P06239 Related reagents
P06241 Related reagents
Entrez Gene
LCK Related reagents
FYN Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0001948 glycoprotein binding
GO:0005829 cytosol
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0005768 endosome
GO:0006468 protein phosphorylation
GO:0006816 calcium ion transport
GO:0007243 intracellular protein kinase cascade
GO:0007411 axon guidance
GO:0007612 learning
GO:0007631 feeding behavior
GO:0042802 identical protein binding
GO:0016032 viral reproduction
GO:0030168 platelet activation
GO:0031295 T cell costimulation
GO:0044419 interspecies interaction between organisms
GO:0050690 regulation of defense response to virus by virus
GO:0050852 T cell receptor signaling pathway
GO:0050900 leukocyte migration
Regulatory
For research purposes only

Applications of Lck (pTyr416)/FYN (pTyr416) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Boggon, T.J. & Eck, M.J. (2004) Structure and regulation of Src family kinases.
    Oncogene. 23 (48): 7918-27.
  2. Irtegun, S. et al. (2013) Tyrosine 416 is phosphorylated in the closed, repressed conformation of c-Src.
    PLoS One. 8 (7): e71035.