LAT1 antibody

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Rabbit anti Human LAT1:Preservative Free

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1827Z P 0.1 mg
Rabbit anti Human LAT1 antibody recognizes human LAT1 (L-type amino acid transporter 1), a heterodimeric Na+-independent amino acid transporter protein and member of the amino acid-polyamine-organocation (APC) superfamily, involved in the high-affinity transport of large neutral amino acids, in association with SLC3A2/4F2hc.

LAT1 is highly expressed in the brain and is involved in the transport of L-DOPA across the blood-brain barrier, in brain neurogenesis, and acts as a mediator of L-leucine transport across the inner blood-retinal barrier. LAT1 is involved in the cell membrane transport of the thyroid hormones triiodothyronine (T3) and thyroxine (T4) in the placenta, and is the foremost transporter of tyrosine in fibroblasts.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human Lat1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Keyhole limpet haemocyanin (KLH) conjugated synthetic peptide C-RHRKPEL, representing an internal portion of human LAT1 located towards the C-terminus (aa. 419-425).
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q01650 Related reagents
Entrez Gene
SLC7A5 Related reagents
GO Terms
GO:0007596 blood coagulation
GO:0016021 integral to membrane
GO:0005829 cytosol
GO:0042605 peptide antigen binding
GO:0006520 cellular amino acid metabolic process
GO:0006811 ion transport
GO:0007399 nervous system development
GO:0015175 neutral amino acid transmembrane transporter activity
GO:0016324 apical plasma membrane
GO:0030154 cell differentiation
GO:0050900 leukocyte migration
Regulatory
For research purposes only

Applications of LAT1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 5ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Breast

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Product Specific References

References for LAT1 antibody

  1. Fraga, S. et al. (2005) Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells.
    Amino Acids. 29 (3): 229-33.
  2. Soares-da-silva, P. et al. (2005) Expression and function of LAT1, a neutral amino acid exchanger, in renal porcine epithelial cell line LLC-PK.
    Acta Physiol Scand. 185 (1): 71-8.
  3. Pinho, M.J. et al. (2011) LAT1 overexpression and function compensates downregulation of ASCT2 in an in vitro model of renal proximal tubule cell ageing.
    Mol Cell Biochem. 349 (1-2): 107-16.