LAT1 antibody
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Rabbit anti Human LAT1 antibody recognizes human LAT1 (L-type amino acid transporter 1), a heterodimeric Na+-independent amino acid transporter protein and member of the amino acid-polyamine-organocation (APC) superfamily, involved in the high-affinity transport of large neutral amino acids, in association with SLC3A2/4F2hc.
LAT1 is highly expressed in the brain and is involved in the transport of L-DOPA across the blood-brain barrier, in brain neurogenesis, and acts as a mediator of L-leucine transport across the inner blood-retinal barrier. LAT1 is involved in the cell membrane transport of the thyroid hormones triiodothyronine (T3) and thyroxine (T4) in the placenta, and is the foremost transporter of tyrosine in fibroblasts.
LAT1 is highly expressed in the brain and is involved in the transport of L-DOPA across the blood-brain barrier, in brain neurogenesis, and acts as a mediator of L-leucine transport across the inner blood-retinal barrier. LAT1 is involved in the cell membrane transport of the thyroid hormones triiodothyronine (T3) and thyroxine (T4) in the placenta, and is the foremost transporter of tyrosine in fibroblasts.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human Lat1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Keyhole limpet haemocyanin (KLH) conjugated synthetic peptide C-RHRKPEL, representing an internal portion of human LAT1 located towards the C-terminus (aa. 419-425).
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q01650
- Entrez Gene
- SLC7A5
- GO Terms
- GO:0007596 blood coagulation
- GO:0016021 integral to membrane
- GO:0005829 cytosol
- GO:0042605 peptide antigen binding
- GO:0006520 cellular amino acid metabolic process
- GO:0006811 ion transport
- GO:0007399 nervous system development
- GO:0015175 neutral amino acid transmembrane transporter activity
- GO:0016324 apical plasma membrane
- GO:0030154 cell differentiation
- GO:0050900 leukocyte migration
- Regulatory
- For research purposes only
Applications of LAT1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin | 5ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Breast
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
|
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Product Specific References
References for LAT1 antibody
-
Fraga, S. et al. (2005) Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells.
Amino Acids. 29 (3): 229-33. -
Soares-da-silva, P. et al. (2005) Expression and function of LAT1, a neutral amino acid exchanger, in renal porcine epithelial cell line LLC-PK.
Acta Physiol Scand. 185 (1): 71-8. -
Pinho, M.J. et al. (2011) LAT1 overexpression and function compensates downregulation of ASCT2 in an in vitro model of renal proximal tubule cell ageing.
Mol Cell Biochem. 349 (1-2): 107-16.
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