Rabbit anti Human LAT1 antibody recognizes human LAT1 (L-type amino acid transporter 1), a heterodimeric Na+-independent amino acid transporter protein and member of the amino acid-polyamine-organocation (APC) superfamily, involved in the high-affinity transport of large neutral amino acids, in association with SLC3A2/4F2hc.
LAT1 is highly expressed in the brain and is involved in the transport of L-DOPA across the blood-brain barrier, in brain neurogenesis, and acts as a mediator of L-leucine transport across the inner blood-retinal barrier. LAT1 is involved in the cell membrane transport of the thyroid hormones triiodothyronine (T3) and thyroxine (T4) in the placenta, and is the foremost transporter of tyrosine in fibroblasts.
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human Lat1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Keyhole limpet haemocyanin (KLH) conjugated synthetic peptide C-RHRKPEL, representing an internal portion of human LAT1 located towards the C-terminus (aa. 419-425).
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to membrane
peptide antigen binding
cellular amino acid metabolic process
nervous system development
neutral amino acid transmembrane transporter activity
apical plasma membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of LAT1 antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
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Secondary Antibodies Available
Product Specific References
References for LAT1 antibody
Fraga, S. et al. (2005) Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells.
Amino Acids. 29 (3): 229-33.
Soares-da-silva, P. et al. (2005) Expression and function of LAT1, a neutral amino acid exchanger, in renal porcine epithelial cell line LLC-PK.
Acta Physiol Scand. 185 (1): 71-8.
Pinho, M.J. et al. (2011) LAT1 overexpression and function compensates downregulation of ASCT2 in an in vitro model of renal proximal tubule cell ageing.
Mol Cell Biochem. 349 (1-2): 107-16.
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