Laminin antibody
Rabbit anti Human laminin antibody (AHP2491) used for the visualization of laminin in normal colon and in colonic adenocarcinoma tissue sections by immunofluorescence.
Image caption:
Representative indirect immunofluorescence images showing double-stained sections for the detection of the α1 integrin subunit and laminin in a normal colon mucosa (A and B) and a moderately differentiated colon adenocarcinoma specimen (C, D). The α1 subunit (red signal) was observed at the basolateral domain of normal epithelial cells of the lower crypt compartment (LC in panel A and E in panel B) compared to weak signal in the upper compartment (UC, panel A). In CRC, α1 was also localized at the basolateral domain of tumour epithelial cells (E) as well as in the adjacent subepithelial myofibroblasts (MF) (Panels C, D). Basement membrane (arrows) located at the interface between the two tissues was stained with an anti-laminin antibody (green staining). Nuclei were stained with DAPI (blue). Scale bar = 50 μm.
From: Boudjadi S, Carrier JC, Beaulieu JF.
Integrin α1 subunit is up-regulated in colorectal cancer.
Biomark Res. 2013 Mar 7;1(1):16.
Rabbit anti laminin antibody recognizes laminin, also known as LAMB1 or laminin subunit beta-1.
Laminin subunits form the heterotrimeric extracellular matrix protein that is essential for basement membrane assembly and embryonic development.
Laminin subunits form the heterotrimeric extracellular matrix protein that is essential for basement membrane assembly and embryonic development.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to laminin was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Recombinant human laminin
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P07942
- Entrez Gene
- LAMB1
- GO Terms
- GO:0005201 extracellular matrix structural constituent
- GO:0005606 laminin-1 complex
- GO:0005607 laminin-2 complex
- GO:0005615 extracellular space
- GO:0007411 axon guidance
- GO:0030335 positive regulation of cell migration
- GO:0034446 substrate adhesion-dependent cell spreading
- GO:0042476 odontogenesis
- GO:0043257 laminin-8 complex
- GO:0043259 laminin-10 complex
- GO:0048471 perinuclear region of cytoplasm
- GO:0050679 positive regulation of epithelial cell proliferation
- Regulatory
- For research purposes only
Applications of Laminin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | 1/4000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti laminin antibody detects a band of approximately 198 kDa in cell lysates and tissue lysates under reducing conditions
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for Laminin antibody
-
Boudjadi, S. et al. (2013) Integrin α1 subunit is up-regulated in colorectal cancer.
Biomark Res. 1 (1): 16.
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