Lactate Dehydrogenase 5 antibody

Sheep anti Human Lactate Dehydrogenase 5

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Lactate Dehydrogenase 5

Product Code Applications Pack Size List Price Your Price Qty
5603-0036
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 1 ml
List Price Your Price
Search for Batch Specific Datasheets

Sheep anti lactate dehydrogenase 5 antibody reacts with LDH2-5, and exhibits low reactivity with LDH1. L-lactate dehydrogenase isozymes exist as tetramers of two subunits with LDH-1 composed for 4 β chains and LDH-5 composed of 4 α chains. LDH-2 =3β:1α, LDH-3 = 2β:2α, LDH-4 = 1β:3α (Boyer et al. 1963).

Mutations in the LDHA gene can lead to the development of glycogen storage disease 11 (GSD11), a condition typified by myogloinurea, fatigue and pain.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Native, highly purified lactate dehydrogenase 5.
Approx. Protein Concentrations
IgG concentration 5.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 1/100 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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Further Reading

  1. Markert, C.L. (1963) Lactate Dehydrogenase Isozymes: Dissociation and Recombination of Subunits.
    Science. 140 (3573): 1329-30.
  2. Augoff, K. et al. (2015) Lactate dehydrogenase 5: an old friend and a new hope in the war on cancer.
    Cancer Lett. 358 (1): 1-7.
  3. Boyer, S.H. et al. (1963) Lactate dehydrogenase variant from human blood: evidence for molecular subunits.
    Science. 141 (3581): 642-3.

RRID
AB_2137174
UniProt
P00338
Entrez Gene
LDHA
GO Terms
GO:0005515 protein binding
GO:0004459 L-lactate dehydrogenase activity
GO:0005829 cytosol
GO:0006090 pyruvate metabolic process
GO:0006096 glycolysis
GO:0055114 oxidation-reduction process
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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