Lactate Dehydrogenase 5 antibody

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Sheep anti Human Lactate Dehydrogenase 5

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
5603-0036
Datasheet
E WB
MSDS
1 ml

Sheep anti lactate dehydrogenase 5 antibody reacts with LDH2-5, and exhibits low reactivity with LDH1. L-lactate dehydrogenase isozymes exist as tetramers of two subunits with LDH-1 composed for 4 β chains and LDH-5 composed of 4 α chains. LDH-2 =3β:1α, LDH-3 = 2β:2α, LDH-4 = 1β:3α (Boyer et al. 1963).

Mutations in the LDHA gene can lead to the development of glycogen storage disease 11 (GSD11), a condition typified by myogloinurea, fatigue and pain.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Native, highly purified lactate dehydrogenase 5.
Approx. Protein Concentrations
IgG concentration 5.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch

More Information

UniProt
P00338 Related reagents
Entrez Gene
LDHA Related reagents
GO Terms
GO:0005515 protein binding
GO:0004459 L-lactate dehydrogenase activity
GO:0005829 cytosol
GO:0006090 pyruvate metabolic process
GO:0006096 glycolysis
GO:0055114 oxidation-reduction process
Regulatory
For research purposes only

Applications of Lactate Dehydrogenase 5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 1/100 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 C E F WB 1.5 ml loader
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA F IF 0.1 mg loader
Donkey anti Sheep/Goat IgG:FITC STAR88F C 1 mg loader
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader

Product Specific References

Further Reading

  1. Markert, C.L. (1963) Lactate Dehydrogenase Isozymes: Dissociation and Recombination of Subunits.
    Science. 140 (3573): 1329-30.
  2. Augoff, K. et al. (2015) Lactate dehydrogenase 5: an old friend and a new hope in the war on cancer.
    Cancer Lett. 358 (1): 1-7.
  3. Boyer, S.H. et al. (1963) Lactate dehydrogenase variant from human blood: evidence for molecular subunits.
    Science. 141 (3581): 642-3.