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Ku80 antibody

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Western blotting analysis of HeLa cell lysate (35μg protein in RIPA buffer) probed using Goat anti Human Ku80 antibody (AHP1391) at 0.1μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

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Goat anti Human Ku80

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Ku80

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Product Code Applications Pack Size List Price Your Price Qty
AHP1391
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg
List Price Your Price

Goat anti Human Ku80 antibody recognizes the 80 kDa subunit of human Ku protein, an evolutionarily conserved nuclear ATP-dependent DNA helicase, involved in a major proportion of DNA repair and in V(D)J recombination.

The Ku protein, originally described as an autoantigen, exists as a tightly associated heterodimer consisting of a 70 kDa (Ku70) and 80 kDa (Ku80) subunit which binds to DNA double-strand break ends (DSB). DNA bound Ku recruits the large catalytic subunit DNA-PKcs to form the DNA-dependent protein kinase complex DNA-PK, facilitating DNA repair by the non-homologous end-joining (NHEJ) pathway.

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human Ku80 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence C-SLAKKDEKTDTLED from the internal region of Ku80 (NP_066964.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/16,000
Western Blotting 0.1 0.3ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1391 detects a band of approximately 80-85kDa in HeLa cell lysates. An additional 75kDa band of unknown origin may be observed.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):HRP

References for Ku80 antibody

  1. Koike, M. (2002) Dimerization, translocation and localization of Ku70 and Ku80 proteins.
    J Radiat Res. 43 (3): 223-36.
  2. Dedieu, S. et al. (2010) The cytoprotective drug amifostine modifies both expression and activity of the pro-angiogenic factor VEGF-A.
    BMC Med. 8:19.
  3. Slupianek, A. et al. (2011) BCR/ABL Stimulates WRN to Promote Survival and Genomic Instability.
    Cancer Res. 71: 842-51.

Western Blotting

Synonyms
XRCC5
RRID
AB_2218624
UniProt
P13010
Entrez Gene
XRCC5
GO Terms
GO:0000723 telomere maintenance
GO:0000783 nuclear telomere cap complex
GO:0003690 double-stranded DNA binding
GO:0004003 ATP-dependent DNA helicase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006303 double-strand break repair via nonhomologous end joining
GO:0006350 transcription
GO:0042162 telomeric DNA binding
GO:0008022 protein C-terminus binding
GO:0010843 promoter binding
GO:0016481 negative regulation of transcription
GO:0019047 provirus integration
GO:0019059 initiation of viral infection
GO:0043564 Ku70:Ku80 complex
GO:0070419 nonhomologous end joining complex

AHP1391

148911

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