Rabbit anti Ku70 antibody recognizes human Ku70, also known as XRCC6, G22P1 or X-ray repair cross-complementing protein 6.
Ku70 is involved in repair of double stranded DNA breaks via the non-homologous end joining (NHEJ) pathway. Ku70 forms a heterodimer with Ku80 (Ku70/Ku80), which binds to the ends of broken DNA where it recruits proteins for end-processing and ligation.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to Ku70 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Recombinant human Ku70
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
nuclear telomere cap complex
double-stranded DNA binding
ATP-dependent DNA helicase activity
transcription factor complex
DNA-dependent protein kinase-DNA ligase 4 complex
double-strand break repair via nonhomologous end joining
protein C-terminus binding
positive regulation of gene-specific transcription from RNA polymerase II promoter
negative regulation of transcription
transcription activator activity
initiation of viral infection
5'-deoxyribose-5-phosphate lyase activity
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Ku70 antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti Ku70 antibody detects a band of approximately 70 kDa in cell and tissue lysates under reducing conditions
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Secondary Antibodies Available
Useful Reagents Available