Krueppel-Like Factor 4 antibody
Rabbit anti Krueppel-Like Factor 4
Krüeppel-like factor 4 antibody recognizes Krüeppel-like factor 4, also known as KIF4, endothelial Krüppel-like zinc finger protein, epithelial zinc finger protein EZF or gut-enriched Krüeppel-like factor. This antibody has been extensively validated for western blotting using whole cell lysates.
Krueppel-Like Factor 4 Antibody with Control Lysate
Krüeppel-like factor 4 antibody recognizes Krüeppel-like factor 4, also known as KIF4, endothelial Krüppel-like zinc finger protein, epithelial zinc finger protein EZF or gut-enriched Krüeppel-like factor. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00223 | WB | 100 µl | ||||
| VPA00223KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human Krueppel-like factor 4 antibody recognizes Krueppel-like factor 4, also known as endothelial Kruppel-like zinc finger protein, epithelial zinc finger protein EZF and gut-enriched krueppel-like factor.
Rabbit anti Human Krueppel-like factor 4 antibody detects a band of 62 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Rabbit anti Human Krueppel-like factor 4 antibody detects a band of 62 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti Kreuppel-like factor 4 detects a band of approximately 62 kDa in HepG2 cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit Ig fraction prepared by ammonium sulphate precipitation
- Preparation
- 20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH conjugated synthetic peptide between 20-53 amino acids from the N-terminal region of human Krueppel-like factor 4
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HepG2 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- O43474 Related reagents
- Entrez Gene
- KLF4 Related reagents
- GO Terms
- GO:0001010 sequence-specific DNA binding transcription factor recruiting transcription factor activity
- GO:0001085 RNA polymerase II transcription factor binding
- GO:0005634 nucleus
- GO:0007500 mesodermal cell fate determination
- GO:0008270 zinc ion binding
- GO:0008285 negative regulation of cell proliferation
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010843 promoter binding
- GO:0016563 transcription activator activity
- GO:0016564 transcription repressor activity
- GO:0019827 stem cell maintenance
- GO:0032569 gene-specific transcription from RNA polymerase II promoter
- GO:0045444 fat cell differentiation
- GO:0045892 negative regulation of transcription, DNA-dependent
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Krueppel-Like Factor 4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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