Krueppel-Like Factor 4 antibody

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Rabbit anti Krueppel-Like Factor 4

Krüeppel-like factor 4 antibody recognizes Krüeppel-like factor 4, also known as KIF4, endothelial Krüppel-like zinc finger protein, epithelial zinc finger protein EZF or gut-enriched Krüeppel-like factor. This antibody has been extensively validated for western blotting using whole cell lysates.

Krueppel-Like Factor 4 Antibody with Control Lysate

Krüeppel-like factor 4 antibody recognizes Krüeppel-like factor 4, also known as KIF4, endothelial Krüppel-like zinc finger protein, epithelial zinc finger protein EZF or gut-enriched Krüeppel-like factor. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00223 WB 100 µl
VPA00223KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human Krueppel-like factor 4 antibody recognizes Krueppel-like factor 4, also known as endothelial Kruppel-like zinc finger protein, epithelial zinc finger protein EZF and gut-enriched krueppel-like factor.

Rabbit anti Human Krueppel-like factor 4 antibody detects a band of 62 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation
Preparation
20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide between 20-53 amino acids from the N-terminal region of human Krueppel-like factor 4

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
O43474 Related reagents
Entrez Gene
KLF4 Related reagents
GO Terms
GO:0001010 sequence-specific DNA binding transcription factor recruiting transcription factor activity
GO:0001085 RNA polymerase II transcription factor binding
GO:0005634 nucleus
GO:0007500 mesodermal cell fate determination
GO:0008270 zinc ion binding
GO:0008285 negative regulation of cell proliferation
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0016564 transcription repressor activity
GO:0019827 stem cell maintenance
GO:0032569 gene-specific transcription from RNA polymerase II promoter
GO:0045444 fat cell differentiation
GO:0045892 negative regulation of transcription, DNA-dependent
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Krueppel-Like Factor 4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti Kreuppel-like factor 4 detects a band of approximately 62 kDa in HepG2 cell lysates
Western Blotting
Anti Krueppel-like factor 4 detects a band of approximately 62 kDa in HepG2 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB