KPNA4 antibody

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Goat anti KPNA4

Goat anti-human KPNA4 antibody recognizes importin subunit alpha-3, also known as importin alpha Q1, importin subunit alpha-4 or karyopherin subunit alpha-4. This antibody has been extensively validated for western blotting using whole cell lysates.

KPNA4 Antibody with Control Lysate

Goat anti-human KPNA4 antibody recognizes importin subunit alpha-3, also known as importin alpha Q1, importin subunit alpha-4 or karyopherin subunit alpha-4. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00546 WB 100 µl
VPA00546KT WB 2 Westerns
With Lysate 400µg
Goat anti Human KPNA4 antibody recognizes KPNA4 also known as importin subunit alpha-3, importin alpha Q1, importin subunit alpha-4 or karyopherin subunit alpha-4.

The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by KPNA4 gene shares sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen (provided by RefSeq, Jul 2008).

Goat anti Human KPNA4 antibody detects a band of 58 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti KPNA4 detects a band of approximately 58 kDa in NIH/3T3 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Product Form
Purified IgG - liquid.
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution
TRIS buffered saline.
Buffer Solution
TRIS buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin.
Immunogen
Peptide sequence C-NSSANVPTEGFQF
Instructions For Use
Please refer to the PrecisionAb western blotting protocol.

Positive Control Lysate

Lysate Composition
400μg NIH/3T3 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
O00629 Related reagents
Entrez Gene
KPNA4 Related reagents
GO Terms
GO:0005515 protein binding
GO:0005643 nuclear pore
GO:0005737 cytoplasm
GO:0006607 NLS-bearing substrate import into nucleus
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only.

Applications of KPNA4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

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