Kinesin Heavy Chain antibody

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Goat anti Kinesin Heavy Chain

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00053
Datasheet
WB
MSDS
100 µl

Goat anti Human kinesin heavy chain antibody recognizes the heavy chain of human kinesin, also known as kinesin-1 heavy chain, conventional kinesin heavy chain, ubiquitous kinesin heavy chain or KIF5B.

Kinesin heavy chain is a 963 amino acid microtubule dependent motor protein. This protein is a member of the kinesin-like protein family and principal component of kinesin, involved in the plus end-directed transport of eukaryotic cell vesicles and organelles.

Kinesins are microtubule associated motor proteins which consist of 2 identical (60-70 kDa) light chains and 2 identical (110-120 kDa) heavy chains, the latter of which contain a globular C-terminal tail domain, a central alpha helical rod domain and a globular N-terminal motor domain with microtubule-stimulated ATPase activity.

Goat anti Human kinesin heavy chain antibody recognizes kinesin heavy chain as a band of 110 kDa in a wide range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.

Product Details

Target Species
Human
Western Blotting
Goat anti kinesin heavy chain detects a band of approximately 110 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence C-QPVAVRGGGGKQV from the C-terminal region of kinesin heavy chain.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P33176
Entrez Gene
KIF5B
GO Terms
GO:0003777 microtubule motor activity
GO:0005524 ATP binding
GO:0005871 kinesin complex
GO:0005874 microtubule
GO:0008017 microtubule binding
GO:0035617 stress granule disassembly
GO:0047496 vesicle transport along microtubule
GO:0048471 perinuclear region of cytoplasm
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Kinesin Heavy Chain antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader