Kinesin Heavy Chain antibody

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Anti Human Kinesin Heavy Chain (C-Terminal) Antibody thumbnail image 1

Anti Human Kinesin Heavy Chain (C-Terminal) Antibody thumbnail image 2

Anti Human Kinesin Heavy Chain (C-Terminal) Antibody gallery image 1 — Western blotting analysis of lysates of HeLa cell lysate (35μg protein in RIPA buffer) probed using Goat anti Human kinesin heavy chain antibody (**AHP1395**) at 0.03μgml^-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

Anti Human Kinesin Heavy Chain (C-Terminal) Antibody gallery image 2 — Immunohistochemical staining of formalin fixed, paraffin embedded human kidney following heat mediated antigen retrieval in Tris/EDTA buffer pH9.0 using Goat anti Human kinesin heavy chain antibody (**AHP1395**) at 1.0μgml^-1. Visualization was accomplished with peroxidase conjugated secondary antibody and DAB substrate development

Goat anti Human Kinesin Heavy Chain (C-Terminal)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP1395	E P* WB			0.1 mg

Goat anti Human Kinesin heavy chain antibodyrecognizes the heavy chain of human kinesin, also known as Kinesin-1 heavy chain, Conventional kinesin heavy chain, Ubiquitous kinesin heavy chain or KIF5B. Kinesin heavy chain is a 963 amino acid microtubule dependent motor containing a kinesin motor domain. otherwise known as , a member of the kinesin-like protein family and principal component of kinesin, involved in the plus end-directed transport of eukaryotic cell vesicles and organelles. Goat anti human kinesin heavy chain antibody binds to an epitope within the C-terminal (CT) region of human kinesin heavy chain (KHC),

Kinesins are microtubule associated motor proteins which consist of 2 identical (60-70 kDa) light chains and 2 identical (110-120 kDa) heavy chains, the latter of which contain a globular C-terminal tail domain, a central alpha helical rod domain and a globular N-terminal motor domain with microtubule-stimulated ATPase activity.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Dog

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antiserum to human kinesin heavy chain (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Buffer Solution

TRIS buffered saline

Preservative Stabilisers

0.02% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin

Immunogen

Peptide sequence C-QPVAVRGGGGKQV from the C-terminal region of kinesin heavy chain (NP_004512.).

Approx. Protein Concentrations

IgG concentration 0.5mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P33176 Related reagents
Entrez Gene: KIF5B Related reagents
GO Terms: GO:0003777 microtubule motor activity; GO:0005524 ATP binding; GO:0005871 kinesin complex; GO:0005874 microtubule; GO:0008017 microtubule binding; GO:0035617 stress granule disassembly; GO:0047496 vesicle transport along microtubule; GO:0048471 perinuclear region of cytoplasm
Regulatory: For research purposes only

Applications of Kinesin Heavy Chain antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA		1/8,000
Immunohistology - Paraffin ¹	1.0	2.0ug/ml
Western Blotting	0.5	1.5ug/ml

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Human kidney
Western Blotting: AHP1395 detects a band of approximately 120kDa in human kidney cell lysates.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Rabbit anti Goat IgG (Fc):FITC	STAR122F	1 mg	F
Rabbit anti Goat IgG (Fc):HRP	STAR122P	1 mg	C E WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Antigen Retrieval Buffer, pH8.0	BUF025A	500 ml	P
Antigen Retrieval Buffer, pH8.0	BUF025C	50 ml	P