Kinesin Heavy Chain antibody


Kinesins are microtubule associated motor proteins which consist of 2 identical (60-70 kDa) light chains and 2 identical (110-120 kDa) heavy chains, the latter of which contain a globular C-terminal tail domain, a central alpha helical rod domain and a globular N-terminal motor domain with microtubule-stimulated ATPase activity.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Dog - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human kinesin heavy chain (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Peptide sequence C-QPVAVRGGGGKQV from the C-terminal region of kinesin heavy chain (NP_004512.).
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P33176
- Entrez Gene
- KIF5B
- GO Terms
- GO:0003777 microtubule motor activity
- GO:0005524 ATP binding
- GO:0005871 kinesin complex
- GO:0005874 microtubule
- GO:0008017 microtubule binding
- GO:0035617 stress granule disassembly
- GO:0047496 vesicle transport along microtubule
- GO:0048471 perinuclear region of cytoplasm
- Regulatory
- For research purposes only
Applications of Kinesin Heavy Chain antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/8,000 | ||
Immunohistology - Paraffin 1 | 1.0 | 2.0ug/ml | |
Western Blotting | 0.5 | 1.5ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Histology Positive Control Tissue
- Human kidney
- Western Blotting
- AHP1395 detects a band of approximately 120kDa in human kidney cell lysates.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
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Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
Product Specific References
Further Reading
-
Navone, F. et al. (1992) Cloning and expression of a human kinesin heavy chain gene: interaction of the COOH-terminal domain with cytoplasmic microtubules in transfected CV-1 cells.
J Cell Biol. 117 (6): 1263-75.
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