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Kinesin Heavy Chain antibody

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Anti Human Kinesin Heavy Chain (C-Terminal) Antibody thumbnail image 1 Anti Human Kinesin Heavy Chain (C-Terminal) Antibody thumbnail image 2
Anti Human Kinesin Heavy Chain (C-Terminal) Antibody gallery image 1
Western blotting analysis of lysates of HeLa cell lysate (35μg protein in RIPA buffer) probed using Goat anti Human kinesin heavy chain antibody (AHP1395) at 0.03μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.
Anti Human Kinesin Heavy Chain (C-Terminal) Antibody gallery image 2
Immunohistochemical staining of formalin fixed, paraffin embedded human kidney following heat mediated antigen retrieval in Tris/EDTA buffer pH9.0 using Goat anti Human kinesin heavy chain antibody (AHP1395) at 1.0μgml-1. Visualization was accomplished with peroxidase conjugated secondary antibody and DAB substrate development

Goat anti Human Kinesin Heavy Chain (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1395
Datasheet
E P* WB
SDS
0.1 mg

Goat anti Human Kinesin heavy chain antibodyrecognizes the heavy chain of human kinesin, also known as Kinesin-1 heavy chain, Conventional kinesin heavy chain, Ubiquitous kinesin heavy chain or KIF5B. Kinesin heavy chain is a 963 amino acid microtubule dependent motor containing a kinesin motor domain. otherwise known as , a member of the kinesin-like protein family and principal component of kinesin, involved in the plus end-directed transport of eukaryotic cell vesicles and organelles. Goat anti human kinesin heavy chain antibody binds to an epitope within the C-terminal (CT) region of human kinesin heavy chain (KHC),

Kinesins are microtubule associated motor proteins which consist of 2 identical (60-70 kDa) light chains and 2 identical (110-120 kDa) heavy chains, the latter of which contain a globular C-terminal tail domain, a central alpha helical rod domain and a globular N-terminal motor domain with microtubule-stimulated ATPase activity.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human kinesin heavy chain (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence C-QPVAVRGGGGKQV from the C-terminal region of kinesin heavy chain (NP_004512.).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P33176
Entrez Gene
KIF5B
GO Terms
GO:0003777 microtubule motor activity
GO:0005524 ATP binding
GO:0005871 kinesin complex
GO:0005874 microtubule
GO:0008017 microtubule binding
GO:0035617 stress granule disassembly
GO:0047496 vesicle transport along microtubule
GO:0048471 perinuclear region of cytoplasm
Regulatory
For research purposes only

Applications of Kinesin Heavy Chain antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/8,000
Immunohistology - Paraffin 1 1.0 2.0ug/ml
Western Blotting 0.5 1.5ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human kidney
Western Blotting
AHP1395 detects a band of approximately 120kDa in human kidney cell lysates.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml

Product Specific References

Further Reading

  1. Navone, F. et al. (1992) Cloning and expression of a human kinesin heavy chain gene: interaction of the COOH-terminal domain with cytoplasmic microtubules in transfected CV-1 cells.
    J Cell Biol. 117 (6): 1263-75.
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