Kinesin Heavy Chain antibody
Goat anti Human Kinesin heavy chain antibodyrecognizes the heavy chain of human kinesin, also known as Kinesin-1 heavy chain, Conventional kinesin heavy chain, Ubiquitous kinesin heavy chain or KIF5B. Kinesin heavy chain is a 963 amino acid microtubule dependent motor containing a kinesin motor domain. otherwise known as , a member of the kinesin-like protein family and principal component of kinesin, involved in the plus end-directed transport of eukaryotic cell vesicles and organelles. Goat anti human kinesin heavy chain antibody binds to an epitope within the C-terminal (CT) region of human kinesin heavy chain (KHC),
Kinesins are microtubule associated motor proteins which consist of 2 identical (60-70 kDa) light chains and 2 identical (110-120 kDa) heavy chains, the latter of which contain a globular C-terminal tail domain, a central alpha helical rod domain and a globular N-terminal motor domain with microtubule-stimulated ATPase activity.
Kinesins are microtubule associated motor proteins which consist of 2 identical (60-70 kDa) light chains and 2 identical (110-120 kDa) heavy chains, the latter of which contain a globular C-terminal tail domain, a central alpha helical rod domain and a globular N-terminal motor domain with microtubule-stimulated ATPase activity.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Dog - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human kinesin heavy chain (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Peptide sequence C-QPVAVRGGGGKQV from the C-terminal region of kinesin heavy chain (NP_004512.).
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P33176
- Entrez Gene
- KIF5B
- GO Terms
- GO:0003777 microtubule motor activity
- GO:0005524 ATP binding
- GO:0005871 kinesin complex
- GO:0005874 microtubule
- GO:0008017 microtubule binding
- GO:0035617 stress granule disassembly
- GO:0047496 vesicle transport along microtubule
- GO:0048471 perinuclear region of cytoplasm
- Regulatory
- For research purposes only
Applications of Kinesin Heavy Chain antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/8,000 | ||
| Immunohistology - Paraffin 1 | 1.0 | 2.0ug/ml | |
| Western Blotting | 0.5 | 1.5ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human kidney
- Western Blotting
- AHP1395 detects a band of approximately 120kDa in human kidney cell lysates.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
Product Specific References
Further Reading
-
Navone, F. et al. (1992) Cloning and expression of a human kinesin heavy chain gene: interaction of the COOH-terminal domain with cytoplasmic microtubules in transfected CV-1 cells.
J Cell Biol. 117 (6): 1263-75.
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