KGF antibody

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Anti Human KGF Antibody thumbnail image 2

Anti Human KGF Antibody gallery image 1 — Formalin fixed, paraffin embedded normal human skin stained with Rabbit anti Human KGF (**AHP783**) following antigen retrieval using proteinase K

Anti Human KGF Antibody gallery image 2 — Formalin fixed, paraffin embedded normal human skin stained with Rabbit anti Human KGF (**AHP783**) following antigen retrieval using proteinase K

Rabbit anti Human KGF

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP783	E FN P* WB			0.1 mg

Rabbit anti Human KGF antibody recognizes human KGF (keratinocyte growth factor), a member of the fibroblast growth factor family, which acts as a potent mitogen of epithelial cells, predominantly of keratinocyte origin.

KGF is secreted by fibroblasts in response to stimulation from inflammatory mediators, including IL-1 beta and PDGF-BB (platelet-derived growth factor B), and binds specifically to a splice variant of the FGF receptor 2 (FGFR2-IIIb), known as the KGF receptor.

Product Details

Target Species: Human
Product Form: Purified IgG - lyophilised
Reconstitution: Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Antiserum Preparation: Antisera to human KGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: None present
Immunogen: Recombinant human KGF.
Approx. Protein Concentrations: IgG concentration 1.0mg/ml

Storage Information

Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.

More Information

UniProt: P21781 Related reagents
Entrez Gene: FGF7 Related reagents
GO Terms: GO:0008083 growth factor activity; GO:0008286 insulin receptor signaling pathway; GO:0008543 fibroblast growth factor receptor signaling pathway; GO:0010463 mesenchymal cell proliferation; GO:0010838 positive regulation of keratinocyte proliferation; GO:0031532 actin cytoskeleton reorganization; GO:0034394 protein localization at cell surface; GO:0042056 chemoattractant activity; GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation; GO:0051549 positive regulation of keratinocyte migration; GO:0051781 positive regulation of cell division; GO:0060501 positive regulation of epithelial cell proliferation involved in lung morphogenesis; GO:0061033 secretion by lung epithelial cell involved in lung growth
Regulatory: For research purposes only

Applications of KGF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA		0.5ug/ml
Functional Assays	0.56	0.85ug/ml
Immunohistology - Paraffin ¹
Western Blotting	0.1	0.2ug/ml

¹ This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Sheep anti Rabbit IgG:Biotin	2AB02B	1 ml	C E P WB
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	1 mg	E WB
Goat anti Rabbit IgG (Fc):FITC	STAR121F	1 mg	F
Goat anti Rabbit IgG (Fc):HRP	STAR121P	1 mg	E WB
Goat anti Rabbit IgG (H/L):HRP	STAR124P	1 mg	C E WB
Sheep anti Rabbit IgG:FITC	STAR34B	1 mg	C F
Sheep anti Rabbit IgG:RPE	STAR35A	1 ml	F
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:DyLight®549	STAR36D549GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®649	STAR36D649GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	0.1 mg	F IF WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Antigen Retrieval Buffer, pH8.0	BUF025A	500 ml	P
Antigen Retrieval Buffer, pH8.0	BUF025C	50 ml	P
TidyBlot™ Western Blot Detection Reagent:HRP	STAR209P	0.5 ml	WB*

Product Specific References

References for KGF antibody

Al-hamilly, N.S. et al. (2016) Biological roles of KGF, CTGF and TGF-β in cyclosporine-A- and phenytoin- induced gingival overgrowth: A comparative experimental animal study.
Arch Oral Biol. 66: 38-43.