KGF antibody
Rabbit anti Human KGF antibody recognizes human KGF (keratinocyte growth factor), a member of the fibroblast growth factor family, which acts as a potent mitogen of epithelial cells, predominantly of keratinocyte origin.
KGF is secreted by fibroblasts in response to stimulation from inflammatory mediators, including IL-1 beta and PDGF-BB (platelet-derived growth factor B), and binds specifically to a splice variant of the FGF receptor 2 (FGFR2-IIIb), known as the KGF receptor.
KGF is secreted by fibroblasts in response to stimulation from inflammatory mediators, including IL-1 beta and PDGF-BB (platelet-derived growth factor B), and binds specifically to a splice variant of the FGF receptor 2 (FGFR2-IIIb), known as the KGF receptor.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
- Antiserum Preparation
- Antisera to human KGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human KGF.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P21781
- Entrez Gene
- FGF7
- GO Terms
- GO:0008083 growth factor activity
- GO:0008286 insulin receptor signaling pathway
- GO:0008543 fibroblast growth factor receptor signaling pathway
- GO:0010463 mesenchymal cell proliferation
- GO:0010838 positive regulation of keratinocyte proliferation
- GO:0031532 actin cytoskeleton reorganization
- GO:0034394 protein localization at cell surface
- GO:0042056 chemoattractant activity
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0051549 positive regulation of keratinocyte migration
- GO:0051781 positive regulation of cell division
- GO:0060501 positive regulation of epithelial cell proliferation involved in lung morphogenesis
- GO:0061033 secretion by lung epithelial cell involved in lung growth
- Regulatory
- For research purposes only
Applications of KGF antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.5ug/ml | ||
| Functional Assays | 0.56 | 0.85ug/ml | |
| Immunohistology - Paraffin 1 | |||
| Western Blotting | 0.1 | 0.2ug/ml |
- 1 This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for KGF antibody
-
Al-hamilly, N.S. et al. (2016) Biological roles of KGF, CTGF and TGF-β in cyclosporine-A- and phenytoin- induced gingival overgrowth: A comparative experimental animal study.
Arch Oral Biol. 66: 38-43.
Further Reading
-
Rubin, J.S. et al. (1989) Purification and characterization of a newly identified growth factor specific for epithelial cells.
Proc Natl Acad Sci U S A. 86 (3): 802-6.
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