Rabbit anti Human KGF antibody recognizes human KGF (keratinocyte growth factor), a member of the fibroblast growth factor family, which acts as a potent mitogen of epithelial cells, predominantly of keratinocyte origin.
KGF is secreted by fibroblasts in response to stimulation from inflammatory mediators, including IL-1 beta and PDGF-BB (platelet-derived growth factor B), and binds specifically to a splice variant of the FGF receptor 2 (FGFR2-IIIb), known as the KGF receptor.
- Target Species
- Product Form
- Purified IgG - lyophilised
- Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
- Antiserum Preparation
- Antisera to human KGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Recombinant human KGF.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
growth factor activity
insulin receptor signaling pathway
fibroblast growth factor receptor signaling pathway
mesenchymal cell proliferation
positive regulation of keratinocyte proliferation
actin cytoskeleton reorganization
protein localization at cell surface
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of keratinocyte migration
positive regulation of cell division
positive regulation of epithelial cell proliferation involved in lung morphogenesis
secretion by lung epithelial cell involved in lung growth
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of KGF antibody
|Immunohistology - Paraffin 1
- 1 This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for KGF antibody
Al-hamilly, N.S. et al. (2016) Biological roles of KGF, CTGF and TGF-β in cyclosporine-A- and phenytoin- induced gingival overgrowth: A comparative experimental animal study.
Arch Oral Biol. 66: 38-43.
Rubin, J.S. et al. (1989) Purification and characterization of a newly identified growth factor specific for epithelial cells.
Proc Natl Acad Sci U S A. 86 (3): 802-6.