KGF antibody

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Rabbit anti Human KGF

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP783 E FN P* WB 0.1 mg
Rabbit anti Human KGF antibody recognizes human KGF (keratinocyte growth factor), a member of the fibroblast growth factor family, which acts as a potent mitogen of epithelial cells, predominantly of keratinocyte origin.

KGF is secreted by fibroblasts in response to stimulation from inflammatory mediators, including IL-1 beta and PDGF-BB (platelet-derived growth factor B), and binds specifically to a splice variant of the FGF receptor 2 (FGFR2-IIIb), known as the KGF receptor.

Product Details

Target Species
Human
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Antiserum Preparation
Antisera to human KGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant human KGF.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P21781 Related reagents
Entrez Gene
FGF7 Related reagents
GO Terms
GO:0008083 growth factor activity
GO:0008286 insulin receptor signaling pathway
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0010463 mesenchymal cell proliferation
GO:0010838 positive regulation of keratinocyte proliferation
GO:0031532 actin cytoskeleton reorganization
GO:0034394 protein localization at cell surface
GO:0042056 chemoattractant activity
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0051549 positive regulation of keratinocyte migration
GO:0051781 positive regulation of cell division
GO:0060501 positive regulation of epithelial cell proliferation involved in lung morphogenesis
GO:0061033 secretion by lung epithelial cell involved in lung growth
Regulatory
For research purposes only

Applications of KGF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5ug/ml
Functional Assays 0.56 0.85ug/ml
Immunohistology - Paraffin 1
Western Blotting 0.1 0.2ug/ml
  1. 1 This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for KGF antibody

  1. Al-hamilly, N.S. et al. (2016) Biological roles of KGF, CTGF and TGF-β in cyclosporine-A- and phenytoin- induced gingival overgrowth: A comparative experimental animal study.
    Arch Oral Biol. 66: 38-43.

Further Reading

  1. Rubin, J.S. et al. (1989) Purification and characterization of a newly identified growth factor specific for epithelial cells.
    Proc Natl Acad Sci U S A. 86 (3): 802-6.