IRS1 (pSer636) antibody
Rabbit anti IRS1 (pSer636) antibody recognizes insulin receptor substrate 1 (IRS1), when phosphorylated at serine 636. IRS proteins are substrates of the insulin receptor tyrosine kinase and play a key role in insulin signaling.
IRS1 is phosphorylated at serine 636 by mTOR and p70S6K (Tzatsos 2009, Zhang et al. 2008).
IRS1 is phosphorylated at serine 636 by mTOR and p70S6K (Tzatsos 2009, Zhang et al. 2008).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to IRS1 (pSer636) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding serine 636 of human IRS1
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P35568
- Entrez Gene
- IRS1
- GO Terms
- GO:0005829 cytosol
- GO:0005068 transmembrane receptor protein tyrosine kinase adaptor activity
- GO:0005080 protein kinase C binding
- GO:0005158 insulin receptor binding
- GO:0005159 insulin-like growth factor receptor binding
- GO:0005634 nucleus
- GO:0005792 microsome
- GO:0005899 insulin receptor complex
- GO:0005901 caveola
- GO:0008286 insulin receptor signaling pathway
- GO:0008543 fibroblast growth factor receptor signaling pathway
- GO:0014065 phosphatidylinositol 3-kinase cascade
- GO:0046326 positive regulation of glucose import
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0032000 positive regulation of fatty acid beta-oxidation
- GO:0042169 SH2 domain binding
- GO:0042593 glucose homeostasis
- GO:0043548 phosphatidylinositol 3-kinase binding
- GO:0043552 positive regulation of phosphatidylinositol 3-kinase activity
- GO:0045725 positive regulation of glycogen biosynthetic process
- GO:0046627 negative regulation of insulin receptor signaling pathway
- GO:0046628 positive regulation of insulin receptor signaling pathway
- GO:0046676 negative regulation of insulin secretion
- GO:0048011 nerve growth factor receptor signaling pathway
- Regulatory
- For research purposes only
Applications of IRS1 (pSer636) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | 1/3000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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|
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Tzatsos, A. (2009) Raptor binds the SAIN (Shc and IRS-1 NPXY binding) domain of insulin receptor substrate-1 (IRS-1) and regulates the phosphorylation of IRS-1 at Ser-636/639 by mTOR.
J Biol Chem. 284 (34): 22525-34. -
Zhang, J. et al. (2008) S6K directly phosphorylates IRS-1 on Ser-270 to promote insulin resistance in response to TNF-(alpha) signaling through IKK2.
J Biol Chem. 283 (51): 35375-82.
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