IRS1 (pSer636) antibody

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Rabbit anti IRS1 (pSer636)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP2637	WB			50 µl

Rabbit anti IRS1 (pSer636) antibody recognizes insulin receptor substrate 1 (IRS1), when phosphorylated at serine 636. IRS proteins are substrates of the insulin receptor tyrosine kinase and play a key role in insulin signaling.

IRS1 is phosphorylated at serine 636 by mTOR and p70S6K (Tzatsos 2009, Zhang et al. 2008).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antiserum to IRS1 (pSer636) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.02% Sodium Azide
50% Glycerol

Immunogen

Phospho specific-peptide corresponding to residues surrounding serine 636 of human IRS1

Storage Information

Storage: Store at -20^oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch

More Information

UniProt: P35568 Related reagents
Entrez Gene: IRS1 Related reagents
GO Terms: GO:0005829 cytosol; GO:0005068 transmembrane receptor protein tyrosine kinase adaptor activity; GO:0005080 protein kinase C binding; GO:0005158 insulin receptor binding; GO:0005159 insulin-like growth factor receptor binding; GO:0005634 nucleus; GO:0005792 microsome; GO:0005899 insulin receptor complex; GO:0005901 caveola; GO:0008286 insulin receptor signaling pathway; GO:0008543 fibroblast growth factor receptor signaling pathway; GO:0014065 phosphatidylinositol 3-kinase cascade; GO:0046326 positive regulation of glucose import; GO:0048015 phosphatidylinositol-mediated signaling; GO:0032000 positive regulation of fatty acid beta-oxidation; GO:0042169 SH2 domain binding; GO:0042593 glucose homeostasis; GO:0043548 phosphatidylinositol 3-kinase binding; GO:0043552 positive regulation of phosphatidylinositol 3-kinase activity; GO:0045725 positive regulation of glycogen biosynthetic process; GO:0046627 negative regulation of insulin receptor signaling pathway; GO:0046628 positive regulation of insulin receptor signaling pathway; GO:0046676 negative regulation of insulin secretion; GO:0048011 nerve growth factor receptor signaling pathway
Regulatory: For research purposes only

Applications of IRS1 (pSer636) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000	1/3000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Sheep anti Rabbit IgG:Biotin	2AB02B	1 ml	C E P WB
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	1 mg	E WB
Goat anti Rabbit IgG (Fc):FITC	STAR121F	1 mg	F
Goat anti Rabbit IgG (Fc):HRP	STAR121P	1 mg	E WB
Goat anti Rabbit IgG (H/L):HRP	STAR124P	1 mg	C E WB
Sheep anti Rabbit IgG:FITC	STAR34B	1 mg	C F
Sheep anti Rabbit IgG:RPE	STAR35A	1 ml	F
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:DyLight®549	STAR36D549GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®649	STAR36D649GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	0.1 mg	F IF WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	0.5 ml	WB*