IRS1 (pSer636) antibody
IRS1 is phosphorylated at serine 636 by mTOR and p70S6K (Tzatsos 2009, Zhang et al. 2008).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to IRS1 (pSer636) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding serine 636 of human IRS1
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P35568
- Entrez Gene
- IRS1
- GO Terms
- GO:0005829 cytosol
- GO:0005068 transmembrane receptor protein tyrosine kinase adaptor activity
- GO:0005080 protein kinase C binding
- GO:0005158 insulin receptor binding
- GO:0005159 insulin-like growth factor receptor binding
- GO:0005634 nucleus
- GO:0005792 microsome
- GO:0005899 insulin receptor complex
- GO:0005901 caveola
- GO:0008286 insulin receptor signaling pathway
- GO:0008543 fibroblast growth factor receptor signaling pathway
- GO:0014065 phosphatidylinositol 3-kinase cascade
- GO:0046326 positive regulation of glucose import
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0032000 positive regulation of fatty acid beta-oxidation
- GO:0042169 SH2 domain binding
- GO:0042593 glucose homeostasis
- GO:0043548 phosphatidylinositol 3-kinase binding
- GO:0043552 positive regulation of phosphatidylinositol 3-kinase activity
- GO:0045725 positive regulation of glycogen biosynthetic process
- GO:0046627 negative regulation of insulin receptor signaling pathway
- GO:0046628 positive regulation of insulin receptor signaling pathway
- GO:0046676 negative regulation of insulin secretion
- GO:0048011 nerve growth factor receptor signaling pathway
- Regulatory
- For research purposes only
Applications of IRS1 (pSer636) antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Western Blotting | 1/1000 | 1/3000 |
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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|
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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|
Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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|
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Tzatsos, A. (2009) Raptor binds the SAIN (Shc and IRS-1 NPXY binding) domain of insulin receptor substrate-1 (IRS-1) and regulates the phosphorylation of IRS-1 at Ser-636/639 by mTOR.
J Biol Chem. 284 (34): 22525-34. -
Zhang, J. et al. (2008) S6K directly phosphorylates IRS-1 on Ser-270 to promote insulin resistance in response to TNF-(alpha) signaling through IKK2.
J Biol Chem. 283 (51): 35375-82.
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