Rabbit anti IRS1 (pSer312) antibody
recognizes insulin receptor substrate 1 (IRS1), when phosphorylated at serine 312. IRS proteins are substrates of the insulin receptor tyrosine kinase and play a key role in insulin signaling.
IRS1 is phosphorylated at serine 312 by MAPK8/JNK1 and IKK, inhibiting insulin signaling (Zhang et al. 2008
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to IRS1 (pSer312) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine 312 of human IRS1
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
transmembrane receptor protein tyrosine kinase adaptor activity
protein kinase C binding
insulin receptor binding
insulin-like growth factor receptor binding
insulin receptor complex
insulin receptor signaling pathway
fibroblast growth factor receptor signaling pathway
phosphatidylinositol 3-kinase cascade
positive regulation of glucose import
positive regulation of fatty acid beta-oxidation
SH2 domain binding
phosphatidylinositol 3-kinase binding
positive regulation of phosphatidylinositol 3-kinase activity
positive regulation of glycogen biosynthetic process
negative regulation of insulin receptor signaling pathway
positive regulation of insulin receptor signaling pathway
negative regulation of insulin secretion
nerve growth factor receptor signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IRS1 (pSer312) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Zhang, J. et al. (2008) S6K directly phosphorylates IRS-1 on Ser-270 to promote insulin resistance in response to TNF-(alpha) signaling through IKK2.
J Biol Chem. 283 (51): 35375-82.
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