IRS1 antibody

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Rabbit anti IRS1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP3005
Datasheet
P WB
MSDS
50 µl loader

Rabbit anti IRS1 antibody recognizes insulin receptor substrate 1 (IRS1), also known as HIRS-1.

IRS1 is phosphorylated by insulin receptor tyrosine kinase, and suppression is linked to insulin resistance and type II diabetes (Guo 2014). Nuclear IRS1 appears to contribute to malignant transformation (Reiss et al. 2012). It has been possible to predict onset of Alzheimer's Disease by quantifying a specific phosphorylated form of IRS1 which alters its function, reducing insulin signaling (Kapogiannis et al. 2015).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to IRS1 was raised by repeated immunization of rabbits with highly purified antigen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Recombinant protein of human IRS1

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P35568
Entrez Gene
IRS1
GO Terms
GO:0005829 cytosol
GO:0005068 transmembrane receptor protein tyrosine kinase adaptor activity
GO:0005080 protein kinase C binding
GO:0005158 insulin receptor binding
GO:0005159 insulin-like growth factor receptor binding
GO:0005634 nucleus
GO:0005792 microsome
GO:0005899 insulin receptor complex
GO:0005901 caveola
GO:0008286 insulin receptor signaling pathway
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0014065 phosphatidylinositol 3-kinase cascade
GO:0046326 positive regulation of glucose import
GO:0048015 phosphatidylinositol-mediated signaling
GO:0032000 positive regulation of fatty acid beta-oxidation
GO:0042169 SH2 domain binding
GO:0042593 glucose homeostasis
GO:0043548 phosphatidylinositol 3-kinase binding
GO:0043552 positive regulation of phosphatidylinositol 3-kinase activity
GO:0045725 positive regulation of glycogen biosynthetic process
GO:0046627 negative regulation of insulin receptor signaling pathway
GO:0046628 positive regulation of insulin receptor signaling pathway
GO:0046676 negative regulation of insulin secretion
GO:0048011 nerve growth factor receptor signaling pathway
Regulatory
For research purposes only

Applications of IRS1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/50 1/100
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg