IRP1 antibody
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WB ResetRabbit anti IRP1
- Product Type
- PrecisionAb Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
- Specificity
- IRP1
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| Rabbit anti Human IRP1 antibody recognizes IRP1, also known as IRE-BP 1, citrate hydro-lyase, ACO1 and cytoplasmic aconitate hydratase. The protein encoded by ACO1 is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. Alternative splicing results in multiple transcript variants (provided by RefSeq, May 2013). Rabbit anti Human IRP1 antibody detects a band of 98 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
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- Target Species
- Human
- Western Blotting
- Anti IRP1 antibody recognizes a band of approximately 98 kDa in HCT116 cell lysates
- Species Cross-Reactivity
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Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
<50% Glycerol - Immunogen
- Recombinant protein of human IRP1
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | WB | 2 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
- UniProt
- P21399
- Entrez Gene
- ACO1
- GO Terms
- GO:0005515 protein binding
- GO:0003994 aconitate hydratase activity
- GO:0005829 cytosol
- GO:0005783 endoplasmic reticulum
- GO:0046872 metal ion binding
- GO:0005794 Golgi apparatus
- GO:0006099 tricarboxylic acid cycle
- GO:0006101 citrate metabolic process
- GO:0010040 response to iron(II) ion
- View More GO Terms
- GO:0030350 iron-responsive element binding
- GO:0051539 4 iron, 4 sulfur cluster binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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