IRF5 antibody

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Anti IRF5 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Goat anti Human IRF5 antibody (**VPA00066**) followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 101 second exposure. Arrow points to IRF5 (molecular weight 60 kDa)

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Goat anti IRF5

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: IRF5

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VPA00066 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Goat anti Human IRF5 antibody recognizes interferon regulatory factor 5, a transcription factor belonging to the interferon regulatory factor. IRFs contain a conserved N-terminal region which recognizes the interferon consensus sequence in the promoter region on interferon genes. IRF5 plays a role in M1 macrophage polarization ( Krausgruber et al. , 2011).

Goat anti human IRF5 antibody detects IRF5 as a single band of 60 kDa in a wide range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.

Target Species: Human
Western Blotting: Goat anti IRF5 detects a band of approximately 60 kDa in K562 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution: TRIS buffered saline.
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin
Immunogen: Synthetic peptide sequence C-QGPWPMHPAGMQ from the C terminal region of IRF5.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

UniProt: Q13568
Entrez Gene: IRF5
GO Terms: GO:0003677 DNA binding; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0005634 nucleus; GO:0006355 regulation of transcription, DNA-dependent; GO:0060333 interferon-gamma-mediated signaling pathway; GO:0060337 type I interferon-mediated signaling pathway