Rabbit anri IRE1p antibody recognizes the C-terminus of inositol-requiring kinase 1 precursor (IRE1p), a 108 kDa serine-threonine kinase/endoribonuclease also known as ERN1. It is an endoplasmic reticulum (ER) transmembrane protein, normally bound to the ER molecular chaperones GRP78 and GRP94. At times of ER stress, due to accumulation of unfolded proteins, IRE1p becomes dissociated from the chaperones, which allows it to activate the un-folded protein transcriptional response (UPR). In the mammalian genome, there are two homologs Ire1alpha and Ire1beta. Ire1alpha is expressed in all cells and tissues, Ire1beta expression is primarily restricted to intestinal epithelial cells.
- Target Species
- Human
- Species Cross-Reactivity
-
| Target Species | Cross Reactivity |
|---|
| Rat |
|
| Mouse |
|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to IRE1p were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Immunogen
- A 16 amino acid peptide near the carboxy terminus of human IRE1p.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- UniProt
-
O75460
Related reagents
- Entrez Gene
-
ERN1
Related reagents
- GO Terms
-
GO:0005515
protein binding
-
GO:0000287
magnesium ion binding
-
GO:0006915
apoptosis
-
GO:0004674
protein serine/threonine kinase activity
-
GO:0005524
ATP binding
-
GO:0006350
transcription
-
GO:0006397
mRNA processing
-
GO:0006917
induction of apoptosis
-
GO:0006987
activation of signaling protein activity involved in unfolded protein response
-
GO:0007050
cell cycle arrest
-
GO:0016891
endoribonuclease activity, producing 5'-phosphomonoesters
-
GO:0030176
integral to endoplasmic reticulum membrane
-
GO:0045449
regulation of transcription
- Regulatory
- For research purposes only
Applications of IRE1p antibody
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name |
Verified |
Min Dilution |
Max Dilution |
| Immunohistology - Frozen |
|
|
|
| Western Blotting |
|
1 |
2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1012 detects a band of approximately 110kDa in A-20 cell lysate.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Further Reading
-
Zhang, K. & Kaufman, R.J. (2004) Signaling the unfolded protein response from the endoplasmic reticulum.
J Biol Chem. 279 (25): 25935-8.
-
Schröder, M. et al. (2003) IRE1- and HAC1-independent transcriptional regulation in the unfolded protein response of yeast.
Mol Microbiol. 49 (3): 591-606.
-
Tirasophon, W. et al. (1998) A stress response pathway from the endoplasmic reticulum to the nucleus requires a novel bifunctional protein kinase/endoribonuclease (Ire1p) in mammalian cells.
Genes Dev. 12 (12): 1812-24.
