Rabbit anri IRE1p antibody recognizes the C-terminus of inositol-requiring kinase 1 precursor (IRE1p), a 108 kDa serine-threonine kinase/endoribonuclease also known as ERN1. It is an endoplasmic reticulum (ER) transmembrane protein, normally bound to the ER molecular chaperones GRP78 and GRP94. At times of ER stress, due to accumulation of unfolded proteins, IRE1p becomes dissociated from the chaperones, which allows it to activate the un-folded protein transcriptional response (UPR). In the mammalian genome, there are two homologs Ire1alpha and Ire1beta. Ire1alpha is expressed in all cells and tissues, Ire1beta expression is primarily restricted to intestinal epithelial cells.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to IRE1p were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- A 16 amino acid peptide near the carboxy terminus of human IRE1p.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
magnesium ion binding
protein serine/threonine kinase activity
induction of apoptosis
activation of signaling protein activity involved in unfolded protein response
cell cycle arrest
endoribonuclease activity, producing 5'-phosphomonoesters
integral to endoplasmic reticulum membrane
regulation of transcription
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IRE1p antibody
|Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1012 detects a band of approximately 110kDa in A-20 cell lysate.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Zhang, K. & Kaufman, R.J. (2004) Signaling the unfolded protein response from the endoplasmic reticulum.
J Biol Chem. 279 (25): 25935-8.
Schröder, M. et al. (2003) IRE1- and HAC1-independent transcriptional regulation in the unfolded protein response of yeast.
Mol Microbiol. 49 (3): 591-606.
Tirasophon, W. et al. (1998) A stress response pathway from the endoplasmic reticulum to the nucleus requires a novel bifunctional protein kinase/endoribonuclease (Ire1p) in mammalian cells.
Genes Dev. 12 (12): 1812-24.
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