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IRE1p antibody

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Anti IRE1p (C-Terminal) Antibody thumbnail image 1 Anti IRE1p (C-Terminal) Antibody thumbnail image 2
Anti IRE1p (C-Terminal) Antibody gallery image 1
Western blot analysis of whole cell lysate from A-20 mouse B cell lymphoma cells probed with Rabbit anti Human IRE1p (AHP1012) at 0.5(A), 1(B) and 2(C) ug/ml
Anti IRE1p (C-Terminal) Antibody gallery image 2
Immunocytochemical staining of A-20 cels with rabbit anti Human IRE1p (AHP1012)

Rabbit anti IRE1p (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1012
Datasheet
C WB
SDS
0.1 mg loader

Rabbit anri IRE1p antibody recognizes the C-terminus of inositol-requiring kinase 1 precursor (IRE1p), a 108 kDa serine-threonine kinase/endoribonuclease also known as ERN1. It is an endoplasmic reticulum (ER) transmembrane protein, normally bound to the ER molecular chaperones GRP78 and GRP94. At times of ER stress, due to accumulation of unfolded proteins, IRE1p becomes dissociated from the chaperones, which allows it to activate the un-folded protein transcriptional response (UPR). In the mammalian genome, there are two homologs Ire1alpha and Ire1beta. Ire1alpha is expressed in all cells and tissues, Ire1beta expression is primarily restricted to intestinal epithelial cells.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to IRE1p were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
Immunogen
A 16 amino acid peptide near the carboxy terminus of human IRE1p.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
O75460
Entrez Gene
ERN1
GO Terms
GO:0005515 protein binding
GO:0000287 magnesium ion binding
GO:0006915 apoptosis
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0006350 transcription
GO:0006397 mRNA processing
GO:0006917 induction of apoptosis
GO:0006987 activation of signaling protein activity involved in unfolded protein response
GO:0007050 cell cycle arrest
GO:0016891 endoribonuclease activity, producing 5'-phosphomonoesters
GO:0030176 integral to endoplasmic reticulum membrane
GO:0045449 regulation of transcription
Regulatory
For research purposes only

Applications of IRE1p antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Western Blotting 1 2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1012 detects a band of approximately 110kDa in A-20 cell lysate.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Zhang, K. & Kaufman, R.J. (2004) Signaling the unfolded protein response from the endoplasmic reticulum.
    J Biol Chem. 279 (25): 25935-8.
  2. Schröder, M. et al. (2003) IRE1- and HAC1-independent transcriptional regulation in the unfolded protein response of yeast.
    Mol Microbiol. 49 (3): 591-606.
  3. Tirasophon, W. et al. (1998) A stress response pathway from the endoplasmic reticulum to the nucleus requires a novel bifunctional protein kinase/endoribonuclease (Ire1p) in mammalian cells.
    Genes Dev. 12 (12): 1812-24.

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