IRAK3 antibody

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Anti Human IRAK3 Antibody thumbnail image 2

Anti Human IRAK3 Antibody gallery image 1 — Western blot analysis of lysates from mouse spleen (M) and rat liver (R) probed with Rabbit anti Human IRAK3 (**AHP1611**).

Anti Human IRAK3 Antibody gallery image 2 — Immunocytochemical staining of rat liver with Rabbit anti Human IRAK3 (**AHP1611**).

Rabbit anti Human IRAK3

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP1611	P* WB			0.1 mg

Rabbit anti Human IRAK3 antibody recognizes human Interleukin-1 receptor-associated kinase 3 (IRAK3), a member of the IRAK/Pelle family. IRAKs associate with the IL1/Toll receptors after IL-1 or LPS stimulation, thereby playing an important role in the inflammatory response.

IRAK3 inhibits the dissociation of IRAK1 and IRAK4 from the Toll-like receptor signalling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilising the receptor complex. It is predominantly expressed in peripheral blood lymphocytes. Defects in the IRAK3 gene are associated with susceptibility to asthma-related traits type 5.

Rabbit anti Human IRAK3 antibody does not cross-react with IRAK or IRAK2.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to human IRAK3 were raised by repeated immunisation of rabbits with highly purified antigen.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.02%

Sodium Azide

Immunogen

Peptide corresponding to amino acids from the carboxy terminus of human IRAK3.

Approx. Protein Concentrations

IgG concentration 1 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: Q9Y616 Related reagents
Entrez Gene: IRAK3 Related reagents
GO Terms: GO:0000287 magnesium ion binding; GO:0001960 negative regulation of cytokine-mediated signaling pathway; GO:0002755 MyD88-dependent toll-like receptor signaling pathway; GO:0009615 response to virus; GO:0004674 protein serine/threonine kinase activity; GO:0005524 ATP binding; GO:0005634 nucleus; GO:0005737 cytoplasm; GO:0010933 positive regulation of macrophage tolerance induction; GO:0010936 negative regulation of macrophage cytokine production; GO:0032088 negative regulation of NF-kappaB transcription factor activity; GO:0032494 response to peptidoglycan; GO:0032496 response to lipopolysaccharide; GO:0032695 negative regulation of interleukin-12 production; GO:0032715 negative regulation of interleukin-6 production; GO:0032720 negative regulation of tumor necrosis factor production; GO:0034122 negative regulation of toll-like receptor signaling pathway; GO:0042177 negative regulation of protein catabolic process; GO:0042803 protein homodimerization activity; GO:0043242 negative regulation of protein complex disassembly; GO:0043330 response to exogenous dsRNA; GO:0043407 negative regulation of MAP kinase activity; GO:0045824 negative regulation of innate immune response; GO:0046982 protein heterodimerization activity; GO:0051092 positive regulation of NF-kappaB transcription factor activity; GO:0070498 interleukin-1-mediated signaling pathway; GO:0070555 response to interleukin-1
Regulatory: For research purposes only

Applications of IRAK3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Paraffin ¹			2ug/ml
Western Blotting		0.5	1ug/ml

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP1611 detects a band of approximately 68kDa in mouse spleen lysates.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Sheep anti Rabbit IgG:Biotin	2AB02B	1 ml	C E P WB
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	1 mg	E WB
Goat anti Rabbit IgG (Fc):FITC	STAR121F	1 mg	F
Goat anti Rabbit IgG (Fc):HRP	STAR121P	1 mg	E WB
Goat anti Rabbit IgG (H/L):HRP	STAR124P	1 mg	C E WB
Sheep anti Rabbit IgG:FITC	STAR34B	1 mg	C F
Sheep anti Rabbit IgG:RPE	STAR35A	1 ml	F
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:DyLight®549	STAR36D549GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®649	STAR36D649GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	0.1 mg	F IF WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Antigen Retrieval Buffer, pH8.0	BUF025A	500 ml	P
Antigen Retrieval Buffer, pH8.0	BUF025C	50 ml	P
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	0.5 ml	WB*

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