IRAK3 antibody

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Rabbit anti Human IRAK3

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1611 P* WB 0.1 mg
Rabbit anti Human IRAK3 antibody recognizes human Interleukin-1 receptor-associated kinase 3 (IRAK3), a member of the IRAK/Pelle family. IRAKs associate with the IL1/Toll receptors after IL-1 or LPS stimulation, thereby playing an important role in the inflammatory response.

IRAK3 inhibits the dissociation of IRAK1 and IRAK4 from the Toll-like receptor signalling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilising the receptor complex. It is predominantly expressed in peripheral blood lymphocytes. Defects in the IRAK3 gene are associated with susceptibility to asthma-related traits type 5.

Rabbit anti Human IRAK3 antibody does not cross-react with IRAK or IRAK2.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human IRAK3 were raised by repeated immunisation of rabbits with highly purified antigen.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
Peptide corresponding to amino acids from the carboxy terminus of human IRAK3.
Approx. Protein Concentrations
IgG concentration 1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9Y616 Related reagents
Entrez Gene
IRAK3 Related reagents
GO Terms
GO:0000287 magnesium ion binding
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0009615 response to virus
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0010933 positive regulation of macrophage tolerance induction
GO:0010936 negative regulation of macrophage cytokine production
GO:0032088 negative regulation of NF-kappaB transcription factor activity
GO:0032494 response to peptidoglycan
GO:0032496 response to lipopolysaccharide
GO:0032695 negative regulation of interleukin-12 production
GO:0032715 negative regulation of interleukin-6 production
GO:0032720 negative regulation of tumor necrosis factor production
GO:0034122 negative regulation of toll-like receptor signaling pathway
GO:0042177 negative regulation of protein catabolic process
GO:0042803 protein homodimerization activity
GO:0043242 negative regulation of protein complex disassembly
GO:0043330 response to exogenous dsRNA
GO:0043407 negative regulation of MAP kinase activity
GO:0045824 negative regulation of innate immune response
GO:0046982 protein heterodimerization activity
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0070498 interleukin-1-mediated signaling pathway
GO:0070555 response to interleukin-1
Regulatory
For research purposes only

Applications of IRAK3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 2ug/ml
Western Blotting 0.5 1ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1611 detects a band of approximately 68kDa in mouse spleen lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*