IRAK2 antibody

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Rabbit anti IRAK2

IRAK2 antibody recognizes interleukin-1 receptor-associated kinase-like 2, also known as IRAK-2. The antibody has been extensively validated for western blotting using whole cell lysates.

IRAK2 Antibody with Control Lysate

IRAK2 antibody recognizes interleukin-1 receptor-associated kinase-like 2, also known as IRAK-2. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00134 WB 100 µl
VPA00134KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human IRAK2 antibody recognizes interleukin-1 receptor-associated kinase-like 2 (IRAK2).

IRAK2 is one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL-1R) upon stimulation and is reported to participate in the IL-1-induced upregulation of NF-kappaB (provided by RefSeq, Jul 2008).

Rabbit anti Human IRAK2 antibody detects a band of 69 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti IRAK2 detects a band of approximately 69 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
Peptide corresponding to amino acids near the carboxy terminus of human IRAK2.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
O43187 Related reagents
Entrez Gene
IRAK2 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0000187 activation of MAPK activity
GO:0001959 regulation of cytokine-mediated signaling pathway
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005829 cytosol
GO:0004704 NF-kappaB-inducing kinase activity
GO:0005524 ATP binding
GO:0006954 inflammatory response
GO:0007249 I-kappaB kinase/NF-kappaB cascade
GO:0007254 JNK cascade
GO:0008063 Toll signaling pathway
GO:0009986 cell surface
GO:0010008 endosome membrane
GO:0032088 negative regulation of NF-kappaB transcription factor activity
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0042803 protein homodimerization activity
GO:0045087 innate immune response
GO:0046982 protein heterodimerization activity
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051403 stress-activated MAPK cascade
GO:0070498 interleukin-1-mediated signaling pathway
GO:0070555 response to interleukin-1
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of IRAK2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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