IQGAP1 antibody

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Goat anti IQGAP1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00054
Datasheet
WB
MSDS
100 µl

Goat anti Human IQGAP1 antibody recognizes human IQGAP1, a member of the IQGAP family. IQGAP1 is scaffold protein of, among others, the MAPK pathway, binding three consecutive signaling molecules RAF, MEK and ERK and thereby facilitating their interaction. Through this role in a core signaling network, IQGAP has major impact on cytoskeletal reorganization, proliferation, cell motility and invasion (White et al., 2012).

Researchers have identified IQGAP1 as important to the hyperactive MAPK signaling associated with several forms of cancer ( Jameson et al., 2013).

Goat anti Human IQGAP1 detects IQGAP1 as a band of 189 kDa in a range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.

Product Details

Target Species
Human
Western Blotting
Goat anti IQGAP1 detects a band of approximately 189 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence C-KRQSGQTDPLQKEE, from the internal region of the protein sequence.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P46940
Entrez Gene
IQGAP1
GO Terms
GO:0005886 plasma membrane
GO:0005095 GTPase inhibitor activity
GO:0005099 Ras GTPase activator activity
GO:0005516 calmodulin binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005884 actin filament
GO:0006112 energy reserve metabolic process
GO:0007264 small GTPase mediated signal transduction
GO:0050796 regulation of insulin secretion
GO:0019903 protein phosphatase binding
GO:0030496 midbody
GO:0051056 regulation of small GTPase mediated signal transduction
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of IQGAP1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader