IQGAP1 antibody

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Anti IQGAP1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with IQGAP1 antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 43 second exposure. Arrow points to IQGAP1 (molecular weight 189 kDa)

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Goat anti IQGAP1

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: IQGAP1

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Goat anti Human IQGAP1 antibody recognizes human IQGAP1, a member of the IQGAP family. IQGAP1 is scaffold protein of, among others, the MAPK pathway, binding three consecutive signaling molecules RAF, MEK and ERK and thereby facilitating their interaction. Through this role in a core signaling network, IQGAP has major impact on cytoskeletal reorganization, proliferation, cell motility and invasion (White et al., 2012).

Researchers have identified IQGAP1 as important to the hyperactive MAPK signaling associated with several forms of cancer ( Jameson et al., 2013).

Goat anti Human IQGAP1 detects IQGAP1 as a band of 189 kDa in a range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.

Target Species: Human
Western Blotting: Goat anti IQGAP1 detects a band of approximately 189 kDa in HeLa cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution: TRIS buffered saline.
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin.
Immunogen: Peptide sequence C-KRQSGQTDPLQKEE, from the internal region of the protein sequence.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

UniProt: P46940
Entrez Gene: IQGAP1
GO Terms: GO:0005886 plasma membrane; GO:0005095 GTPase inhibitor activity; GO:0005099 Ras GTPase activator activity; GO:0005516 calmodulin binding; GO:0005634 nucleus; GO:0005737 cytoplasm; GO:0005884 actin filament; GO:0006112 energy reserve metabolic process; GO:0007264 small GTPase mediated signal transduction; View More GO Terms; GO:0050796 regulation of insulin secretion; GO:0019903 protein phosphatase binding; GO:0030496 midbody; GO:0051056 regulation of small GTPase mediated signal transduction