IQGAP1 antibody

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Goat anti IQGAP1

IQGAP1 antibody recognizes Ras GTPase-activating-like protein IQGAP1, also known as p195. The antibody has been extensively validated for western blotting using whole cell lysates.

IQGAP1 Antibody with Control Lysate

IQGAP1 antibody recognizes Ras GTPase-activating-like protein IQGAP1, also known as p195. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00054 WB 100 µl
VPA00054KT WB 2 Westerns
With Lysate 400µg
Goat anti Human IQGAP1 antibody recognizes human IQGAP1, a member of the IQGAP family. IQGAP1 is scaffold protein of, among others, the MAPK pathway, binding three consecutive signaling molecules RAF, MEK and ERK and thereby facilitating their interaction. Through this role in a core signaling network, IQGAP has major impact on cytoskeletal reorganization, proliferation, cell motility and invasion (White et al., 2012).

Researchers have identified IQGAP1 as important to the hyperactive MAPK signaling associated with several forms of cancer ( Jameson et al., 2013).

Goat anti Human IQGAP1 detects IQGAP1 as a band of 189 kDa in a range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.

Product Details

Target Species
Human
Western Blotting
Goat anti IQGAP1 detects a band of approximately 189 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence C-KRQSGQTDPLQKEE, from the internal region of the protein sequence.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P46940 Related reagents
Entrez Gene
IQGAP1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005095 GTPase inhibitor activity
GO:0005099 Ras GTPase activator activity
GO:0005516 calmodulin binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005884 actin filament
GO:0006112 energy reserve metabolic process
GO:0007264 small GTPase mediated signal transduction
GO:0050796 regulation of insulin secretion
GO:0019903 protein phosphatase binding
GO:0030496 midbody
GO:0051056 regulation of small GTPase mediated signal transduction
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of IQGAP1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

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