Rabbit anti IP3R 3 antibody
recognizes inositol 1,4,5-trisphosphate receptor type 3, also known as IP3R 3, InsP3R3 and type 3 inositol 1,4,5-trisphosphate receptor. It is an intracellular Ca2+ release channel that modulates cellular functions including gene expression and cellular growth (Patterson et al. 2004
). The F-box protein FBXL2 binds IP3R3 and targets it for ubiquitin-, p97- and proteasome-mediated degradation. This limits Ca2+ influx into mitochondria (Kuchay et al. 2017
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to IP3R 3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Recombinant fusion protein containing a sequence corresponding to amino acids 1630-1870 of human IP3R 3
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IP3R 3 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti IP3R 3 antibody detects a band of approximately 300 kDa in HeLa, L02 and HepG2 cell lysates under reducing conditions
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
How to Use the Spectraviewer?
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra