IP3R 3 antibody

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Anti IP3R 3 Antibody gallery image 1 — Western blot analysis of HeLa, L02 and HepG2 cell lysates using Rabbit anti IP3R 3 antibody at a 1/3000 dilution. 3% non-fat dry milk in TBST was used for blocking

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Rabbit anti IP3R 3

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: IP3R 3

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AHP2730 Datasheet Datasheet SDS SDS	WB	50 µl		Log in	Get Quote
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Rabbit anti IP3R 3 antibody recognizes inositol 1,4,5-trisphosphate receptor type 3, also known as IP3R 3, InsP3R3 and type 3 inositol 1,4,5-trisphosphate receptor. It is an intracellular Ca2+ release channel that modulates cellular functions including gene expression and cellular growth (Patterson et al. 2004). The F-box protein FBXL2 binds IP3R3 and targets it for ubiquitin-, p97- and proteasome-mediated degradation. This limits Ca2+ influx into mitochondria (Kuchay et al. 2017).

Target Species: Human
Product Form: Purified IgG - liquid
Antiserum Preparation: Antiserum to IP3R 3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.02% Sodium Azide
50% Glycerol
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1630-1870 of human IP3R 3
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Store at -20^oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/500	1/3000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: Rabbit anti IP3R 3 antibody detects a band of approximately 300 kDa in HeLa, L02 and HepG2 cell lysates under reducing conditions

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