Inhibin Alpha antibody

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Rabbit anti Human Inhibin Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
0100-0549 E P 1 ml
Rabbit anti Human inhibin alpha antibody recognizes the human inhibin alpha chain, the product of the INHA gene. The inhibin alpha chain is a 366 amino acid polypeptide with a predicted molecular weight of ~40 kDa. It is composed of an N-terminal 18 amino acid signal peptide, two pro-peptide regions of 43 and 171 amino acids respectively and the 134 amino acid inhibin alpha chain. Inhibin alpha dimerizes with with inhibin beta A to form Inhibin A or with the inhibin beta B chain to form inhibin B. The active inhibin alpha chain is derived by cleavage of the inhibin alpha chain from the pro-peptide domains and subsequent dimerization through appropriate disulphide bond formation. Inhibins regulate follicle stimulating hormone secretion from the pituitary gland (Lumpkin et al. 1981).

Inhibin alpha is expressed in the testis (Bergh and Cajander 1990) and ovary (Cuevas et al. 1987, Yamoto et al. 1992). Inhibin alpha expression has also been noted in the adrenal glands (McCluggage et al. 1998), endomertium (Mylonas et al. 2004) and brain (Uccella et al. 2000). Inhibin alpha expression has also been noted in a variety of neooplastic tissues including Leydig cell tumors (de Winter et al. 1992), brain tumors (Demura et al. 1995), ovarian cancers (Cooke et al. 1995) and adrenal carcinomas (Pelkey et al. 1998).

Rabbit anti human inhibin alpha antibody has been utilized successfully for the immunohistochemical localization of inhibin alpha in benign ovarian Brenner tumor (Kuhn et al. 2013).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide, STPLMSWPWSPSALRLLQRPPEEP, corresponding to the amino acids 1 - 24 of the active cleaved human inhibin alpha chain.
Approx. Protein Concentrations
IgG concentration 5.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P05111 Related reagents
Entrez Gene
INHA Related reagents
GO Terms
GO:0001501 skeletal system development
GO:0001541 ovarian follicle development
GO:0004871 signal transducer activity
GO:0005125 cytokine activity
GO:0005179 hormone activity
GO:0006917 induction of apoptosis
GO:0007050 cell cycle arrest
GO:0007166 cell surface receptor linked signaling pathway
GO:0007267 cell-cell signaling
GO:0007399 nervous system development
GO:0008083 growth factor activity
GO:0009605 response to external stimulus
GO:0046881 positive regulation of follicle-stimulating hormone secretion
GO:0046882 negative regulation of follicle-stimulating hormone secretion
GO:0030218 erythrocyte differentiation
GO:0034673 inhibin-betaglycan-ActRII complex
GO:0042127 regulation of cell proliferation
GO:0042326 negative regulation of phosphorylation
GO:0042541 hemoglobin biosynthetic process
GO:0043512 inhibin A complex
GO:0045077 negative regulation of interferon-gamma biosynthetic process
GO:0045578 negative regulation of B cell differentiation
GO:0045650 negative regulation of macrophage differentiation
Regulatory
For research purposes only

Applications of Inhibin Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1/100 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Histology Positive Control Tissue
Human ovarian cortex

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P

Product Specific References

References for Inhibin Alpha antibody

  1. Kuhn, E. et al. (2013) Ovarian Brenner tumour: a morphologic and immunohistochemical analysis suggesting an origin from fallopian tube epithelium.
    Eur J Cancer. 49 (18): 3839-49.