Rabbit anti Human inhibin alpha antibody
recognizes the human inhibin alpha chain, the product of the INHA gene. The inhibin alpha chain is a 366 amino acid polypeptide with a predicted molecular weight of ~40 kDa. It is composed of an N-terminal 18 amino acid signal peptide, two pro-peptide regions of 43 and 171 amino acids respectively and the 134 amino acid inhibin alpha chain. Inhibin alpha dimerizes with with inhibin beta A
to form Inhibin A or with the inhibin beta B chain to form inhibin B. The active inhibin alpha chain is derived by cleavage of the inhibin alpha chain from the pro-peptide domains and subsequent dimerization through appropriate disulphide bond formation. Inhibins regulate follicle stimulating hormone secretion from the pituitary gland (Lumpkin et al. 1981).
Inhibin alpha is expressed in the testis (Bergh and Cajander 1990) and ovary (Cuevas et al. 1987, Yamoto et al. 1992). Inhibin alpha expression has also been noted in the adrenal glands (McCluggage et al. 1998), endomertium (Mylonas et al. 2004) and brain (Uccella et al. 2000). Inhibin alpha expression has also been noted in a variety of neooplastic tissues including Leydig cell tumors (de Winter et al. 1992), brain tumors (Demura et al. 1995), ovarian cancers (Cooke et al. 1995) and adrenal carcinomas (Pelkey et al. 1998).
Rabbit anti human inhibin alpha antibody has been utilized successfully for the immunohistochemical localization of inhibin alpha in benign ovarian Brenner tumor (Kuhn et al. 2013).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Synthetic peptide, STPLMSWPWSPSALRLLQRPPEEP, corresponding to the amino acids 1 - 24 of the active cleaved human inhibin alpha chain.
- Approx. Protein Concentrations
- IgG concentration 5.0 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
skeletal system development
ovarian follicle development
signal transducer activity
induction of apoptosis
cell cycle arrest
cell surface receptor linked signaling pathway
nervous system development
growth factor activity
response to external stimulus
positive regulation of follicle-stimulating hormone secretion
negative regulation of follicle-stimulating hormone secretion
regulation of cell proliferation
negative regulation of phosphorylation
hemoglobin biosynthetic process
inhibin A complex
negative regulation of interferon-gamma biosynthetic process
negative regulation of B cell differentiation
negative regulation of macrophage differentiation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Inhibin Alpha antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human ovarian cortex
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for Inhibin Alpha antibody
Kuhn, E. et al. (2013) Ovarian Brenner tumour: a morphologic and immunohistochemical analysis suggesting an origin from fallopian tube epithelium.
Eur J Cancer. 49 (18): 3839-49.